Human Coagulation Factor III/Tissue Factor Alexa Fluor® 700-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 323519
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
    Gly34-Glu251
    Accession # P13726
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Coagulation Factor III/Tissue Factor in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human Coagulation Factor III/Tissue Factor Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB23391N) or (B) Mouse IgG1 Alexa Fluor 700 Isotype Control (Catalog # IC002N). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a 46 kDa type I transmembrane protein that is part of the extrinsic pathway of coagulation. It functions as a protein cofactor/receptor for Coagulation Factor VII, which is synthesized in the liver and circulates in the plasma (1). Upon binding to TF, the inactive Factor VII is rapidly converted into activated Factor VIIa. The resulting 1:1 complex of Factor VIIa and TF activates the coagulation pathway, and, as a soluble form, plays an important coagulation-independent role in processes such as angiogenesis and M1 macrophage development (2-5). TF is synthesized as a 295 amino acid (aa) precursor that consists of a signal peptide (aa 1-32) plus a mature domain (aa 33-295). The mature region contain an extracellular region (aa 33-251), followed by a transmembrane segment (aa 252-274) and a cytoplasmic tail (aa 275-295) (6-9). TF is found on endothelial cells, monocytes, vascular smooth muscle cells, and bronchial epithelium (5, 10-12). Over aa 33-251, human and mouse TF share 58% aa sequence identity. 

  • References:
    1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
    2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
    3. Gedding, J.E. and N. Mackman (2014) Thromb. Haemost. 111:570.
    4. Hobbs, J.E. et al. (2007) Thromb. Res. 120 Suppl 2:S13.
    5. Venkatasubramanian, S,  et al. (2015) Eur. J. Immunol. Oct 16:e201545817.
    6. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
    7. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
    8. Morrissey, J.H. et al. (1987) Cell 50:129.
    9. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
    10. Yang, X. et al. (2016) Thromb. Res. 137:211.
    11. Arderiu, G. et al. (2015) Arterioscler. Thromb. Vasc. Biol. 35:348.
    12. Mitchel, J.A. et al. (2015) Am. J. Resp. Cell Mol. Biol. Sept 25 [ePub ahead of print].
  • Entrez Gene IDs:
    2152 (Human); 14066 (Mouse)
  • Alternate Names:
    CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
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