Human Collagen XXIII  alpha 1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4165R-025
Detection  of Collagen XXIII  alpha 1 in NS0 Mouse Cell Line Transfected with Human  Collagen XXIII   alpha 1 by Flow Cytometry.
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Human Collagen XXIII  alpha 1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Collagen XXIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human COL25A1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Collagen XXIII alpha 1
Glu111-Lys540
Accession # Q86Y22
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of Collagen XXIII  alpha 1 in NS0 Mouse Cell Line Transfected with Human  Collagen XXIII   alpha 1 by Flow Cytometry. View Larger

Detection of Collagen XXIII  alpha 1 in NS0 Mouse Cell Line Transfected with Human Collagen XXIII alpha 1 by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Collagen XXIII alpha 1 was stained with Sheep Anti-Human Collagen XXIII  alpha 1 Alexa Fluor® 647‑conjugated Antigen Affinity-purified Polyclonal Antibody
(Catalog # IC4165R, filled histogram) or isotype control antibody (Catalog # IC016R, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Collagen XXIII alpha 1

Collagen XXIII alpha 1 (sometimes abbreviated COL23A1) is a ~75 kDa type II transmembrane nonfibrillar collagen that is a member of the collagenous transmembrane protein superfamily (1, 2). This family also includes collagens XIII, XVII, XXV and non‑collagens with triple‑helical regions such as ectodysplasin A, class A macrophage scavenger receptors, and MARCO (2, 3). The human Collagen XXIII mRNA encodes a 540 amino acid (aa) protein containing a 34 aa N‑terminal cytoplasmic domain, a 21 aa transmembrane (TM) domain and a 485 aa extracellular domain (ECD). The ECD contains a coiled‑coil consensus sequence to aid homotrimerization (aa 64‑69), a furin cleavage site (aa 105‑110), a pair of cysteines thought to form intermolecular disulfides (aa 106 and 108), and three collagen domains (1, 3‑5). The C‑terminal 20 aa, including cysteines at aa 525 and 537 of Collagen XXIII, is conserved among TM collagen proteins. Proteolytic cleavage, occurrs mainly in the Golgi, and allows the Collagen XXIII ectodomain to be secreted as a soluble trimer of ~60 kDa subunits (1, 6). Cell surface cleavage can also occur, but is slow, presumably due to the presence of Collagen XXIII in lipid raft membrane domains (6). The protein database includes three variants of 537, 316 and 309 aa with various portions missing or substituted; all appear to lack TM segments (7). The human Collagen XXIII ECD shares 92%, 93%, and 91% aa sequence identity with mouse, rat, and canine Collagen XXIII, respectively. Collagen XXIII is concentrated at sites of cell contact in epithelia, and is now known to bind to Integrin alpha 2 beta 1 on the surface of stratum basale keratinocytes (2, 5, 8).

References
  1. Banyard, J. et al. (2003) J. Biol. Chem. 278:20989.
  2. Franzke, C-W. et al. (2005) J. Biol. Chem. 280:4005.
  3. Ricard-Blum, S. et al. (2011) Cold Spring Harb. Prespect. Biol. 3:a004978.
  4. Snellman, A. et al. (2007) J. Biol. Chem. 282:14898.
  5. Koch, M. et al. (2006) J. Biol. Chem. 281:21546.
  6. Veit, G. et al. (2007) J. Biol. Chem. 282:27424.
  7. Entrez Accession # EAW53833, EAW53834, and AAH42428.
  8. Veit, G. et al. (2011) J. Biol. Chem. 286:27804.
Entrez Gene IDs
91522 (Human); 237759 (Mouse); 353303 (Rat)
Alternate Names
COL23A1; collagen alpha-1(XXIII) chain; Collagen XXIII alpha 1; collagen, type XXIII, alpha 1; DKFZp434K0621; procollagen, type XXIII, alpha 1

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