Sheep IgG Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
IgG Isotype Control Control
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Product Details
Supplemental Products

Sheep IgG Alexa Fluor® 647-conjugated Antibody Summary

Serum was obtained from naive (nonimmunized) sheep.
Polyclonal Sheep IgG
Protein A or G purified
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 647


Recommended Concentration
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 5 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of Sheep IgGControl by Flow Cytometry NS0 mouse myeloma cell line transfected with human Collagen XXIII alpha 1 wasstained with Sheep Anti-Human Collagen XXIII  alpha 1Alexa Fluor® 647‑conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog #IC4165R,filled histogram) or Sheep IgG Alexa Fluor® 647-conjugated Isotype Control Antibody(Catalog # IC016R, open histogram). To facilitate intracellular staining, cells werefixed and permeabilized with FlowX FoxP3 Fixation &Permeabilization Buffer Kit (Catalog # FC012).View our protocol for StainingIntracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets


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