Sheep IgG Alexa Fluor® 647-conjugated Antibody

  
  • Specificity
    Serum was obtained from naive (nonimmunized) sheep.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Protein A or G purified
  • Immunogen
    NA
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 5 μL/106 cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Sheep IgGControl by Flow Cytometry NS0 mouse myeloma cell line transfected with human Collagen XXIII alpha 1 wasstained with Sheep Anti-Human Collagen XXIII  alpha 1Alexa Fluor® 647‑conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog #IC4165R,filled histogram) or Sheep IgG Alexa Fluor® 647-conjugated Isotype Control Antibody(Catalog # IC016R, open histogram). To facilitate intracellular staining, cells werefixed and permeabilized with FlowX FoxP3 Fixation &Permeabilization Buffer Kit (Catalog # FC012).View our protocol for StainingIntracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Sheep IgG APC-conjugated Antibody

Ctrl IC016A 1  
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Sheep IgG PE-conjugated Antibody

Ctrl IC016P  
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Normal Sheep IgG Fluorescein Control

Ctrl IC016F  
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Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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Sheep IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC016G  
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Sheep IgG Alexa Fluor® 700‑conjugated Control Antibody

Ctrl IC016N  
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