|Detection of Sheep IgGControl by Flow Cytometry NS0 mouse myeloma cell line transfected with human Collagen XXIII alpha 1 wasstained with Sheep Anti-Human Collagen XXIII alpha 1Alexa Fluor® 647‑conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog #IC4165R,filled histogram) or Sheep IgG Alexa Fluor® 647-conjugated Isotype Control Antibody(Catalog # IC016R, open histogram). To facilitate intracellular staining, cells werefixed and permeabilized with FlowX FoxP3 Fixation &Permeabilization Buffer Kit (Catalog # FC012).View our protocol for StainingIntracellular Molecules.|
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
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