Human Complement Component C3a R APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Complement Component C3a R in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 534625
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human C3a R
    Accession # Q16581
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Complement Component C3a R in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human Complement Component C3a R APC‑conjugated Monoclonal Antibody (Catalog # FAB5995A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Complement Component C3a R
Complement Component 3a receptor (C3a R) is a 70-80 kDa G-protein coupled 7-transmembrane sulfated glycoprotein that belongs to the G-protein coupled receptor 1 family of proteins. It is expressed inducibly or constitutively by platelets, eosinophils, monocytes, astrocytes, CD4+ T cells, dendritic cells, NK cells, mast cells and basophils, neutrophils, adipocytes, osteoclasts, osteoblasts macrophages, vascular endothelial cells, bronchial epithelium and smooth muscle. Engagement of C3a R by  77 kDa C3a has a number of effects that are context-dependent. C3a action on adipocytes facilitates glucose and fatty acid uptake, while in bone, C3a R (plus C5aR) drive the generation of Th1 and Th17 cells. Perhaps its best action is that of a mediator of anaphylaxis. On mast cells it induces histamine release, visceral smooth muscle contraction and vasodilation. Human C3a R extracellular regions share 50% and 42% amino acid identity with mouse and rat C3a R, respectively.

  • Long Name:
    Complement Component C3a Receptor
  • Entrez Gene IDs:
    719 (Human); 12267 (Mouse)
  • Alternate Names:
    AZ3B; AZ3BC3a anaphylatoxin chemotactic receptor; C3AR; C3a-R; C3AR1; C3ARcomplement component 3 receptor 1; C3R1; complement component 3a receptor 1; Complement Component C3a R; Complement Component C3aR; HNFAG09
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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