Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro-atrial natriuretic peptide (pro-ANP) to ANP.
Human Corin Quantikine ELISA Kit
R&D Systems | Catalog # DCRN00
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
23.7 pg/mL
Assay Range
75-4800 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human Corin Quantikine ELISA Kit
The Quantikine Human Corin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Corin in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant human Corin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Corin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Corin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human Corin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 618 | 1327 | 2794 | 660 | 1378 | 2819 |
| Standard Deviation | 16.9 | 35.6 | 69.6 | 41.4 | 61.3 | 106.4 |
| CV% | 2.7 | 2.7 | 2.5 | 6.3 | 4.5 | 3.8 |
Recovery for Human Corin Quantikine ELISA Kit
The recovery of Corin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=6) | 96 | 88-101 |
| EDTA Plasma (n=6) | 94 | 87-99 |
| Heparin Plasma (n=6) | 97 | 88-107 |
| Serum (n=6) | 94 | 89-100 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Corin Quantikine ELISA Kit
Human Corin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Corin
Additional Corin Products
Product Documents for Human Corin Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Corin Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human Corin Quantikine ELISA Kit
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Protocols
View specific protocols for Human Corin Quantikine ELISA Kit (DCRN00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
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