Human Corin Quantikine ELISA Kit

  (2 citations)     
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Product Details
Assay Procedure
Citations (2)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    23.7 pg/mL
  • Assay Range
    75.0 - 4,800 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Corin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC67 , Quantikine Immunoassay Control Set 841 for Human Corin - Please Inquire
Product Summary
The Quantikine Human Corin Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Corin in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant human Corin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Corin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Corin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 618 1327 2794 660 1378 2819
Standard Deviation 16.9 35.6 69.6 41.4 61.3 106.4
CV% 2.7 2.7 2.5 6.3 4.5 3.8

Recovery

The recovery of Corin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=6) 96 88-101
EDTA Plasma (n=6) 94 87-99
Heparin Plasma (n=6) 97 88-107
Serum (n=6) 94 89-100
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Corin were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Corin [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Corin
Corin is a type II transmembrane serine protease expressed predominantly in cardiomyocytes. It converts pro-atrial natriuretic peptide (pro-ANP) to ANP.
  • Entrez Gene IDs:
    10699 (Human)
  • Alternate Names:
    ATC2; atrial natriuteric peptide-converting enzyme; Corin; corin, serine peptidase; EC 3.4.21; EC 3.4.21.-; heart specific serine proteinase; Heart-specific serine proteinase ATC2; MGC119742; pro-ANP-convertase; Pro-ANP-Converting Enzyme; PRSC; serine protease; Transmembrane protease serine 10
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Species
Sample Type
  1. Serum Soluble Corin Deficiency Predicts Major Disability within 3 Months after Acute Stroke
    PLoS ONE, 2016;11(9):e0163731.
    Species: Human
    Sample Type: Serum
  2. Plasma soluble corin in patients with heart failure.
    Authors: Dong N, Chen S, Yang J
    Circ Heart Fail, 2010;3(2):207-11.
    Species: Human
    Sample Type: Plasma

FAQs

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ELISA Controls
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Quantikine Immunoassay Control Set 841 for Human Corin

Ctrl QC67
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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