Human CRACC/SLAMF7 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1906N-025
Detection of CRACC/SLAMF7 in Human PBMCs by Flow Cytometry.
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Human CRACC/SLAMF7 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRACC/SLAMF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NTB-AR is observed.
Source
Monoclonal Mouse IgG2A Clone # 235614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRACC/SLAMF7
Lys27-Ser225
Accession # Q9NQ25
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CRACC/SLAMF7 in Human PBMCs by Flow Cytometry. View Larger

Detection of CRACC/SLAMF7 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CRACC/SLAMF7 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1906N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CRACC/SLAMF7

CRACC, also known as CSI (CD2 subset I) and CD319, is a type I transmembrane protein belonging to the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8+ cells, CD4+ cells and B cells. CRACC may play a role in the activation and effector function of T cells and NK cells and likely participates in B cell adhesion. Over amino acids (aa) 27-225, human and mouse SLAMF7 share 53% aa sequence identity.

Entrez Gene IDs
57823 (Human); 75345 (Mouse)
Alternate Names
19A; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD319 antigen; CD319; CRACC; CRACCCD2-like receptor-activating cytotoxic cells; CS119A24 protein; Membrane protein FOAP-12; novel LY9 (lymphocyte antigen 9) like protein; Novel Ly9; Protein 19A; SLAM family member 7; SLAMF7

Product Datasheets

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