Human CD3 epsilon PE-conjugated Antibody

Clone UCHT1 was used by HLDA to establish CD designation
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Detection of CD3 epsilon  in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (2)
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Human CD3 epsilon PE-conjugated Antibody Summary

Species Reactivity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Monoclonal Mouse IgG1 Clone # UCHT1
Protein A or G purified from hybridoma culture supernatant
Human thymocytes (1)
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD3e antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha -beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2). 

  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
  2. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23: 101
Entrez Gene IDs
916 (Human); 12501 (Mouse); 102133065 (Cynomolgus Monkey)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citations for Human CD3 epsilon PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Assessing the expression of immunosuppressive cytokines in the newly diagnosed systemic lupus Erythematosus patients: a focus on B cells
    Authors: M Abbasifard, Z Kamiab, M Hasani, A Rahnama, P Saeed-Aska, H Khorramdel
    BMC Immunol, 2020-11-16;21(1):58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Oxidation of ovarian epithelial cancer cells by hypochlorous acid enhances immunogenicity and stimulates T cells that recognize autologous primary tumor.
    Authors: Chiang CL, Ledermann JA, Aitkens E, Benjamin E, Katz DR, Chain BM
    Clin. Cancer Res., 2008-08-01;14(15):4898-907.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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