Human CD3 epsilon PE-conjugated Antibody

Clone UCHT1 was used by HLDA to establish CD designation.
(1 citations)   
  • Species Reactivity
  • Specificity
    Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
  • Source
    Monoclonal Mouse IgG1 Clone # UCHT1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human thymocytes (1)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD3 epsilon
CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha -beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2). 
  • References:
    1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
    2. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23: 101
  • Entrez Gene IDs:
    916 (Human); 12503 (Mouse)
  • Alternate Names:
    CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Oxidation of ovarian epithelial cancer cells by hypochlorous acid enhances immunogenicity and stimulates T cells that recognize autologous primary tumor.
    Authors: Chiang CL, Ledermann JA, Aitkens E, Benjamin E, Katz DR, Chain BM
    Clin. Cancer Res., 2008;14(15):4898-907.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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