Human CXCL1/2/3/GRO Pan Specific Alexa Fluor® 700-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL1/2/3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) CXCL2 is observed and no cross-reactivity with recombinant human (rh) VIC, rmVIC, rhCCL3L1, rhCCL4L1, or recombinant canine IL-8 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 29702
  • Immunogen
    E. coli-derived recombinant human CXCL3
    Ala35-Asn107
    Accession # P19876
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    0.25-1 µg/106 cells
    Human peripheral blood mononuclear cells treated with PHA, fixed with paraformaldehyde, and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL1/2/3/GRO
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha ) and GRO gamma (MIP-2 beta ), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
  • Alternate Names:
    CXCL1/2/3
Related Research Areas

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