Human CXCL1/2/3/GRO Pan Specific Alexa Fluor® 750-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2761S-100UG
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Human CXCL1/2/3/GRO Pan Specific Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL1/2/3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) CXCL2 is observed and no cross-reactivity with recombinant human (rh) VIC, rmVIC, rhCCL3L1, rhCCL4L1, or recombinant canine IL-8 is observed.
Source
Monoclonal Mouse IgG1 Clone # 29702
Immunogen
E. coli-derived recombinant human CXCL3
Ala35-Asn107
Accession # P19876
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood mononuclear cells treated with PHA, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL1/2/3/GRO

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha ) and GRO gamma (MIP-2 beta ), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.

Alternate Names
CXCL1/2/3

Product Datasheets

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