Detects human CXCL1/2/3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) CXCL2 is observed and no cross-reactivity with recombinant human (rh) VIC, rmVIC, rhCCL3L1, rhCCL4L1, or recombinant canine IL-8 is observed.
Monoclonal Mouse IgG1 Clone # 29702
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CXCL3 Ala35-Asn107 Accession # P19876
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human CXCL1/GRO alpha /KC/CINC-1 (Catalog # 275-GR) Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 (Catalog # 276-GB) Recombinant Human CXCL3/GRO gamma /CINC-2/DCIP-1 (Catalog # 277-GG) under non-reducing conditions only
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with PHA, fixed with paraformaldehyde, and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha )
and GRO gamma (MIP-2 beta ), are members of the CXC subfamily of chemokines.
Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor
type B and are potent neutrophil attractants and activators.
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