Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Mass Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 29702
View all CXCL1/2/3/GRO Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL3
Ala35-Asn107
Accession # P19876

Specificity

Detects human CXCL1/2/3 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) CXCL2 is observed and no cross-reactivity with recombinant human (rh) VIC, rmVIC, rhCCL3L1, rhCCL4L1, or recombinant canine IL-8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human CXCL1/2/3/GRO Pan Specific Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells treated with PHA, fixed with paraformaldehyde, and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CXCL1/GRO alpha /KC/CINC-1 (Catalog # 275-GR)
Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 (Catalog # 276-GB)
Recombinant Human CXCL3/GRO gamma /CINC-2/DCIP-1 (Catalog # 277-GG)
under non-reducing conditions only

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL1/2/3/GRO

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha ) and GRO gamma (MIP-2 beta ), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.

Alternate Names

FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1

UniProt

P19876

Additional CXCL1/2/3/GRO Products

Product Documents for Human CXCL1/2/3/GRO Pan Specific Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL1/2/3/GRO Pan Specific Antibody

For research use only

Citations for Human CXCL1/2/3/GRO Pan Specific Antibody

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