Human CXCL10/IP-10 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
PDIP100
SIP100
DIP100
Control Products Available
CXCL10/IP-10/CRG-2 ELISA Kit ELISA
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Product Details
Procedure
Citations (96)
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Supplemental Products
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Human CXCL10/IP-10 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Saliva (10 uL), Urine (100 uL)
Sensitivity
4.46 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human IP-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IP-10 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IP-10 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IP-10.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 70.3 174 342 80.7 194 362
Standard Deviation 2.07 5.15 10.74 7.91 13.48 24.27
CV% 2.9 3 3.1 9.8 6.9 6.7

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 67.4 168 334 70.7 182 366
Standard Deviation 3.12 4.99 11.15 6.23 11.12 18.93
CV% 4.6 3 3.3 8.8 6.1 5.2

Recovery

The recovery of IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 88-101
EDTA Plasma (n=5) 99 90-109
Heparin Plasma (n=5) 99 87-113
Serum (n=5) 99 88-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IP-10 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL10/IP-10/CRG-2 ELISA Linearity

Data Examples

Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD5K Standard Curve

Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD6Q Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Entrez Gene IDs:
3627 (Human); 15945 (Mouse)
Alternate Names:
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. Assay Diluent
  4.   For Cell Culture Supernate, Saliva, & Urine Samples: Add 150 µL of Assay Diluent to each well.
    For Serum & Plasma Samples: Add 75 µL of Assay Diluent to each well.

  5. Standard, Control, or Sample
  6.   For Cell Culture Supernate, Saliva, & Urine Samples: Add 100 µL of Standard, control, or sample to each well.
    For Serum & Plasma Samples: Add 75 µL of Standard, control, or sample to each well.
  7.   Cover with a plate sealer, and incubate at room temperature for 2 hours.
  8.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  9. 200 µL Conjugate
  10.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CXCL10/IP-10 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

96 Citations: Showing 1 - 10
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  1. TLR9 polymorphism correlates with immune activation, CD4 decline and plasma IP10 levels in HIV patients
    Authors: A Joshi, EB Punke, T Mehmetoglu, DP Peralta, H Garg
    BMC Infect. Dis., 2019;19(1):56.
    Species: Human
    Sample Types: Plasma
  2. Suppression of STING associated with LKB1 loss in KRAS-driven lung cancer
    Authors: S Kitajima, E Ivanova, S Guo, R Yoshida, M Campisi, SK Sundararam, S Tange, Y Mitsuishi, TC Thai, S Masuda, BP Piel, LM Sholl, PT Kirschmeie, CP Paweletz, H Watanabe, M Yajima, DA Barbie
    Cancer Discov, 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Longitudinal transcriptomic characterization of the immune response to acute hepatitis C virus infection in patients with spontaneous viral clearance
    Authors: BR Rosenberg, M Depla, CA Freije, D Gaucher, S Mazouz, M Boisvert, N Bédard, J Bruneau, CM Rice, NH Shoukry
    PLoS Pathog., 2018;14(9):e1007290.
    Species: Human
    Sample Types: Plasma
  4. Tumor innate immunity primed by specific interferon-stimulated endogenous retroviruses
    Authors: I Cañadas, R Thummalapa, JW Kim, S Kitajima, RW Jenkins, CL Christense, M Campisi, Y Kuang, Y Zhang, E Gjini, G Zhang, T Tian, DR Sen, D Miao, Y Imamura, T Thai, B Piel, H Terai, AR Aref, T Hagan, S Koyama, M Watanabe, H Baba, AE Adeni, CA Lydon, P Tamayo, Z Wei, M Herlyn, TU Barbie, R Uppaluri, LM Sholl, E Sicinska, J Sands, S Rodig, KK Wong, CP Paweletz, H Watanabe, DA Barbie
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. HIV-1 proviral landscapes distinguish posttreatment controllers from noncontrollers
    Authors: R Sharaf, GQ Lee, X Sun, B Etemad, LM Aboukhater, Z Hu, ZL Brumme, E Aga, RJ Bosch, Y Wen, G Namazi, C Gao, EP Acosta, RT Gandhi, JM Jacobson, D Skiest, DM Margolis, R Mitsuyasu, P Volberding, E Connick, DR Kuritzkes, MM Lederman, XG Yu, M Lichterfel, JZ Li
    J. Clin. Invest., 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  6. Systemic DPP4 activity is reduced during primary HIV-1 infection and is associated with intestinal RORC+ CD4+ cell levels: a surrogate marker candidate of HIV-induced intestinal damage
    Authors: MJ Ploquin, A Casrouge, Y Madec, N Noël, B Jacquelin, N Huot, D Duffy, SP Jochems, L Micci, C Lécuroux, F Boufassa, T Booiman, T Garcia-Tel, M Ghislain, RL Grand, O Lambotte, N Kootstra, L Meyer, C Goujard, M Paiardini, ML Albert, M Müller-Tru
    J Int AIDS Soc, 2018;21(7):e25144.
    Species: Human
    Sample Types:
  7. Novel Insights into Staphylococcus aureus Deep Bone Infections: the Involvement of Osteocytes
    Authors: D Yang, AR Wijenayaka, LB Solomon, SM Pederson, DM Findlay, SP Kidd, GJ Atkins
    MBio, 2018;9(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Predictive Value of Serum IFN-? inducible Protein-10 and IFN-?/IL-4 Ratio for Liver Fibrosis Progression in CHB Patients
    Authors: Y Wang, W Yu, C Shen, W Wang, L Zhang, F Liu, H Sun, Y Zhao, H Che, C Zhao
    Sci Rep, 2017;7(0):40404.
    Species: Human
    Sample Types: Serum
  9. The therapeutic HIV Env C5/gp41 vaccine candidate Vacc-C5 induces specific T cell regulation in a phase I/II clinical study
    Authors: K Brekke, M Sommerfelt, M Ökvist, AM Dyrhol-Rii, D Kvale
    BMC Infect. Dis, 2017;17(1):228.
    Species: Human
    Sample Types: Serum
  10. Plasma IP-10 Concentrations Correlate Positively with Viraemia and Inversely with CD4 Counts in Untreated HIV Infection
    Authors: K Mhandire, T Mlambo, LS Zijenah, K Duri, K Mateveke, M Tshabalala, DZ Mhandire, C Musarurwa, PT Wekare, LR Mazengera, HT Matarira, B Stray-Pede
    Open AIDS J, 2017;11(0):24-31.
    Species: Human
    Sample Types: Plasma
  11. Thyroid function in children and adolescents with Hashimoto's thyroiditis after L-thyroxine discontinuation
    Authors: G Radetti, M Salerno, C Guzzetti, M Cappa, A Corrias, A Cassio, G Cesaretti, R Gastaldi, M Rotondi, F Lupi, A Fanolla, G Weber, S Loche
    Endocr Connect, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  12. Development of a novel site-specific pegylated interferon beta for antiviral therapy for chronic hepatitis B
    Authors: M Tsuge, T Uchida, N Hiraga, H Kan, GN Makokha, H Abe-Chayam, D Miki, M Imamura, H Ochi, CN Hayes, R Shimozono, T Iwamura, H Narumi, T Suzuki, M Kainoh, T Taniguchi, K Chayama
    Antimicrob. Agents Chemother., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Impact of hepatitis B vaccination on HBsAg kinetics, interferon-inducible protein 10 level and recurrence of viremia
    Authors: A Shaaban Ha
    Cytokine, 2017;99(0):99-105.
    Species: Human
    Sample Types: Serum
  14. Case-control, exploratory study of cerebrospinal fluid chemokines/cytokines and lymphocyte subsets in childhood Tourette syndrome with positive streptococcal markers
    Authors: MR Pranzatell, ED Tate, TJ Allison
    Cytokine, 2017;96(0):49-53.
    Species: Human
    Sample Types: CSF
  15. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
    Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
    Sci Rep, 2017;7(0):46580.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Impact of early cART on HIV blood and semen compartments at the time of primary infection
    Authors: A Chéret, C Durier, A Mélard, M Ploquin, J Heitzmann, C Lécuroux, V Avettand-F, L David, G Pialoux, JM Chennebaul, M Müller-Tru, C Goujard, C Rouzioux, L Meyer,
    PLoS ONE, 2017;12(7):e0180191.
    Species: Human
    Sample Types: Plasma
  17. Changes in inflammatory biomarkers in HCV-infected patients undergoing direct acting antiviral-containing regimens with or without interferon
    Authors: C Mascia, S Vita, P Zuccalà, R Marocco, T Tieghi, S Savinelli, R Rossi, M Iannetta, I Pozzetto, C Furlan, F Mengoni, CM Mastroiann, V Vullo, M Lichtner
    PLoS ONE, 2017;12(6):e0179400.
    Species: Human
    Sample Types: Plasma
  18. Interferon-alpha treatment rapidly clears Hepatitis E virus infection in humanized mice
    Authors: MDB van de Gar, SD Pas, GW van Oord, L Gama, Y Choi, RA de Man, A Boonstra, T Vanwollegh
    Sci Rep, 2017;7(1):8267.
    Species: Mouse
    Sample Types: Serum
  19. Transcript-activated collagen matrix as sustained mRNA delivery system for bone regeneration
    J Control Release, 2016;239(0):137-148.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Randomized Trial Evaluating the Impact of Ribavirin Mono-Therapy and Double Dosing on Viral Kinetics, Ribavirin Pharmacokinetics and Anemia in Hepatitis C Virus Genotype 1 Infection
    PLoS ONE, 2016;11(5):e0155142.
    Species: Human
    Sample Types: Plasma
  21. Elevated Basal Pre-infection CXCL10 in Plasma and in the Small Intestine after Infection Are Associated with More Rapid HIV/SIV Disease Onset
    PLoS Pathog, 2016;12(8):e1005774.
    Species: Human
    Sample Types: Plasma
  22. Left Ventricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study.
    Authors: Altara R, Gu Y, Struijker-Boudier H, Thijs L, Staessen J, Blankesteijn W
    PLoS ONE, 2015;10(10):e0141394.
    Species: Human
    Sample Types: Plasma
  23. Effective Apical Infection of Differentiated Human Bronchial Epithelial Cells and Induction of Proinflammatory Chemokines by the Highly Pneumotropic Human Adenovirus Type 14p1.
    Authors: Lam E, Ramke M, Warnecke G, Schrepfer S, Kopfnagel V, Dobner T, Heim A
    PLoS ONE, 2015;10(7):e0131201.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
    Authors: Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H
    PLoS Pathog, 2015;11(12):e1005319.
    Species: Human
    Sample Types: Plasma
  25. Small Ubiquitin-like Modifier Alters IFN Response.
    Authors: Maarifi G, Maroui M, Dutrieux J, Dianoux L, Nisole S, Chelbi-Alix M
    J Immunol, 2015;195(5):2312-24.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Vitamin D levels vary during antiviral treatment but are unable to predict treatment outcome in HCV genotype 1 infected patients.
    Authors: Grammatikos G, Lange C, Susser S, Schwendy S, Dikopoulos N, Buggisch P, Encke J, Teuber G, Goeser T, Thimme R, Klinker H, Boecher W, Schulte-Frohlinde E, Penna-Martinez M, Badenhoop K, Zeuzem S, Berg T, Sarrazin C
    PLoS ONE, 2014;9(2):e87974.
    Species: Human
    Sample Types: Serum
  27. Randomized comparison of single dose of recombinant human IL-12 versus placebo for restoration of hematopoiesis and improved survival in rhesus monkeys exposed to lethal radiation.
    Authors: Gluzman-Poltorak Z, Mendonca S, Vainstein V, Kha H, Basile L
    J Hematol Oncol, 2014;7(0):31.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  28. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63.
    Species: Human
    Sample Types: Serum
  29. Anti-interferon beta antibody titers strongly correlate between two bioassays and in vivo biomarker expression, and indicates that a titer of 150 TRU/mL is a biologically functional cut-point.
    Authors: Hermanrud C, Ryner M, Engdahl E, Fogdell-Hahn A
    J Interferon Cytokine Res, 2014;34(7):498-504.
    Species: Human
    Sample Types: Serum
  30. Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring?
    Authors: Castley, Alison, Berry, Cassandr, French, Martyn, Fernandez, Sonia, Krueger, Romano, Nolan, David
    PLoS ONE, 2014;9(12):e115226.
    Species: Human
    Sample Types: Plasma
  31. Tumor necrosis factor (TNF)-alpha induction of CXCL10 in endothelial cells requires protein arginine methyltransferase 5 (PRMT5)-mediated nuclear factor (NF)-kappaB p65 methylation.
    Authors: Harris D, Bandyopadhyay S, Maxwell T, Willard B, DiCorleto P
    J Biol Chem, 2014;289(22):15328-39.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Identification of selective small molecule inhibitors of the nucleotide-binding oligomerization domain 1 (NOD1) signaling pathway.
    Authors: Rickard, David J, Sehon, Clark A, Kasparcova, Viera, Kallal, Lorena A, Haile, Pamela A, Zeng, Xin, Montoute, Monica N, Poore, Derek D, Li, Hu, Wu, Zining, Eidam, Patrick, Emery, John G, Marquis, Robert W, Gough, Peter J, Bertin, John
    PLoS ONE, 2014;9(5):e96737.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. BAFF/APRIL system in pediatric OMS: relation to severity, neuroinflammation, and immunotherapy.
    Authors: Pranzatelli, Michael, Tate, Elizabet, McGee, Nathan R, Travelstead, Anna L, Colliver, Jerry A, Ness, Jayne M, Ransohoff, Richard
    J Neuroinflammation, 2013;10(0):10.
    Species: Human
    Sample Types: Serum
  34. Increased CXCL9 serum levels in hepatitis C-related mixed cryoglobulinemia, with autoimmune thyroiditis, associated with high levels of CXCL10.
    Authors: Antonelli A, Fallahi P, Ferrari S, Colaci M, Giuggioli D, Saraceno G, Benvenga S, Ferri C
    J Interferon Cytokine Res, 2013;33(12):739-45.
    Species: Human
    Sample Types: Serum
  35. Expression of CXCR3 and its ligands CXCL9, -10 and -11 in paediatric opsoclonus-myoclonus syndrome.
    Authors: Pranzatelli M, Tate E, McGee N, Travelstead A, Verhulst S, Ransohoff R
    Clin Exp Immunol, 2013;172(3):427-36.
    Species: Human
    Sample Types: Serum
  36. Cleaved/associated TLR3 represents the primary form of the signaling receptor.
    Authors: Toscano F, Estornes Y, Virard F, Garcia-Cattaneo A, Pierrot A, Vanbervliet B, Bonnin M, Ciancanelli M, Zhang S, Funami K, Seya T, Matsumoto M, Pin J, Casanova J, Renno T, Lebecque S
    J Immunol, 2013;190(2):764-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Human trophectoderm apposition is regulated by interferon gamma-induced protein 10 (IP-10) during early implantation.
    Authors: Sela H, Goldman-Wohl D, Haimov-Kochman R, Greenfield C, Natanson-Yaron S, Hamani Y, Revel A, Lavy Y, Singer O, Yachimovich-Cohen N, Turetsky T, Mandelboim O, Reubinoff B, Yagel S
    Placenta, 2013;34(3):222-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Hypoxia-inducible factor-dependent breast cancer-mesenchymal stem cell bidirectional signaling promotes metastasis.
    Authors: Chaturvedi, Pallavi, Gilkes, Daniele, Wong, Carmen C, Luo, Weibo, Zhang, Huafeng, Wei, Hong, Takano, Naoharu, Schito, Luana, Levchenko, Andre, Semenza, Gregg L
    J Clin Invest, 2013;123(1):189-205.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. A20/TNFAIP3 inhibits NF-kappaB activation induced by the Kaposi's sarcoma-associated herpesvirus vFLIP oncoprotein.
    Authors: Sakakibara S, Espigol-Frigole G, Gasperini P, Uldrick T, Yarchoan R, Tosato G
    Oncogene, 2013;32(10):1223-32.
    Species: Human
    Sample Types: Whole Cells
  40. CXCL10 gene promoter polymorphism -1447A>G correlates with plasma CXCL10 levels and is associated with male susceptibility to cerebral malaria.
    Authors: Wilson N, Driss A, Solomon W, Dickinson-Copeland C, Salifu H, Jain V, Singh N, Stiles J
    PLoS ONE, 2013;8(12):e81329.
    Species: Human
    Sample Types: Plasma
  41. Evaluation of IL-28B Polymorphisms and Serum IP-10 in Hepatitis C Infected Chimpanzees.
    Authors: Verstrepen B, De Groot N, Groothuismink Z, Verschoor E, de Groen R, Bogers W, Janssen H, Mooij P, Bontrop R, Koopman G, Boonstra A
    PLoS ONE, 2012;7(10):e46645.
    Species: Human
    Sample Types: Serum
  42. USP18 is a key regulator of the interferon-driven gene network modulating pancreatic beta cell inflammation and apoptosis.
    Authors: Santin I, Moore F, Grieco F, Marchetti P, Brancolini C, Eizirik D
    Cell Death Dis, 2012;3(0):e419.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011;7(4):e1002016.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Circulating chemokine (CXC motif) ligand (CXCL)9 is increased in aggressive chronic autoimmune thyroiditis, in association with CXCL10.
    Authors: Antonelli A, Ferrari SM, Frascerra S, Galetta F, Franzoni F, Corrado A, Miccoli M, Benvenga S, Paolicchi A, Ferrannini E, Fallahi P
    Cytokine, 2011;55(2):288-93.
    Species: Human
    Sample Types: Serum
  45. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
    J. Invest. Dermatol., 2011;131(1):133-40.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. CD14-independent responses induced by a synthetic lipid A mimetic.
    Authors: Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D
    Eur J Immunol, 2010;40(3):797-802.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway.
    Authors: Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W
    J. Clin. Invest., 2010;120(5):1645-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  48. Synthesis and immunological activities of novel agonists of toll-like receptor 9.
    Authors: Struthers M, Bett AJ, Wisniewski T, Dubey SA, Precopio M, Jiang W, Sun Z, Wang H, Nowak I, Putta MR, Yu D, Tang JX, Kandimalla ER, Agrawal S, Casimiro DR
    Cell. Immunol., 2010;263(1):105-13.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
  49. Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis.
    Authors: Antonelli A, Ferri C, Ferrari SM, De Marco S, Di Domenicantonio A, Centanni M, Pupilli C, Villa E, Menichetti F, Fallahi P
    J. Interferon Cytokine Res., 2010;30(11):835-42.
    Species: Human
    Sample Types: Serum
  50. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
    Species: Human
    Sample Types: Tears
  51. Serum IP-10 as a biomarker of human rhinovirus infection at exacerbation of COPD.
    Authors: Quint JK, Donaldson GC, Goldring JJ, Baghai-Ravary R, Hurst JR, Wedzicha JA
    Chest, 2010;137(4):812-22.
    Species: Human
    Sample Types: Serum
  52. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010;28(5):939-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Cerebrospinal fluid in HIV-1 systemic viral controllers: absence of HIV-1 RNA and intrathecal inflammation.
    Authors: Probasco JC, Deeks SG, Lee E, Hoh R, Hunt PW, Liegler T, Price RW, Spudich SS
    AIDS, 2010;24(7):1001-5.
    Species: Human
    Sample Types: CSF
  54. CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
    Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA
    Eur. Respir. J., 2009;34(3):676-86.
    Species: Human
    Sample Types: BALF
  55. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009;182(6):3919-27.
    Species: Human
    Sample Types: Plasma
  56. Phenotype of atopic dermatitis subjects with a history of eczema herpeticum.
    Authors: Beck LA, Boguniewicz M, Hata T, Schneider LC, Hanifin J, Gallo R, Paller AS, Lieff S, Reese J, Zaccaro D, Milgrom H, Barnes KC, Leung DY
    J. Allergy Clin. Immunol., 2009;124(2):260-9, 269.e1.
    Species: Human
    Sample Types: Serum
  57. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009;2009(0):269541.
    Species: Human
    Sample Types: Serum
  58. The major outer membrane protein of a periodontopathogen induces IFN-beta and IFN-stimulated genes in monocytes via lipid raft and TANK-binding kinase 1/IFN regulatory factor-3.
    Authors: Lee SH, Kim JS, Jun HK
    J. Immunol., 2009;182(9):5823-35.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. CXCL10 and CCL2 chemokine serum levels in patients with hepatitis C associated with autoimmune thyroiditis.
    Authors: Antonelli A, Ferri C, Fallahi P, Ferrari SM, Frascerra S, Pampana A, Panicucci E, Carpi A, Nicolini A, Ferrannini E
    J. Interferon Cytokine Res., 2009;29(6):345-51.
    Species: Human
    Sample Types: Serum
  60. The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma.
    Authors: Linge HM, Collin M, Giwercman A, Malm J, Bjartell A, Egesten A
    J. Interferon Cytokine Res., 2008;28(3):191-6.
    Species: Human
    Sample Types: Seminal Plasma
  61. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Endometrial NK cells are special immature cells that await pregnancy.
    Authors: Manaster I, Mizrahi S, Goldman-Wohl D, Sela HY, Stern-Ginossar N, Lankry D, Gruda R, Hurwitz A, Bdolah Y, Haimov-Kochman R, Yagel S, Mandelboim O
    J. Immunol., 2008;181(3):1869-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Evidence for a pathogenetic role of interleukin-18 in cutaneous lupus erythematosus.
    Authors: Wang D, Drenker M, Eiz-Vesper B, Werfel T, Wittmann M
    Arthritis Rheum., 2008;58(10):3205-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
    Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
    J. Interferon Cytokine Res., 2008;28(5):297-310.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Urinary fractalkine is a marker of acute rejection.
    Authors: Peng W, Chen J, Jiang Y, Wu J, Shou Z, He Q, Wang Y, Chen Y, Wang H
    Kidney Int., 2008;74(11):1454-60.
    Species: Human
    Sample Types: Urine
  66. Agonists of proteinase-activated receptor-2 enhance IFN-gamma-inducible effects on human monocytes: role in influenza A infection.
    Authors: Feld M, Shpacovitch VM, Ehrhardt C, Kerkhoff C, Hollenberg MD, Vergnolle N, Ludwig S, Steinhoff M
    J. Immunol., 2008;180(10):6903-10.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
    Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
    Chest, 2007;133(1):26-33.
    Species: Human
    Sample Types: Saliva
  68. Polymorphisms in the chemokine (C-X-C motif) ligand 10 are associated with invasive aspergillosis after allogeneic stem-cell transplantation and influence CXCL10 expression in monocyte-derived dendritic cells.
    Authors: Mezger M, Steffens M, Beyer M, Manger C, Eberle J, Toliat MR, Wienker TF, Ljungman P, Hebart H, Dornbusch HJ, Einsele H, Loeffler J
    Blood, 2007;111(2):534-6.
    Species: Human
    Sample Types: Serum
  69. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
    Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
    Am. J. Respir. Crit. Care Med., 2007;177(1):44-55.
    Species: Human
    Sample Types: BALF
  70. Phase 2 study of pegylated liposomal doxorubicin in combination with interleukin-12 for AIDS-related Kaposi sarcoma.
    Authors: Little RF, Aleman K, Kumar P, Wyvill KM, Pluda JM, Read-Connole E, Wang V, Pittaluga S, Catanzaro AT, Steinberg SM, Yarchoan R
    Blood, 2007;110(13):4165-71.
    Species: Human
    Sample Types: Serum
  71. Serum interleukin-18 and soluble tumour necrosis factor receptor 2 are associated with disease severity in patients with paracoccidioidomycosis.
    Authors: Corvino CL, Mamoni RL, Fagundes GZ, Blotta MH
    Clin. Exp. Immunol., 2007;147(3):483-90.
    Species: Human
    Sample Types: Serum
  72. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007;111(4):2339-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes.
    Authors: Kanda N, Watanabe S
    Endocrinology, 2007;148(5):2317-25.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
    Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
    J. Immunol., 2007;178(2):1172-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
    Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
    Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. Airway dendritic cell phenotypes in inflammatory diseases of the human lung.
    Authors: Lommatzsch M, Bratke K, Bier A, Julius P, Kuepper M, Luttmann W, Virchow JC
    Eur. Respir. J., 2007;30(5):878-86.
    Species: Human
    Sample Types: BALF
  77. The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cells.
    Authors: Simpkins FA, Devoogdt NM, Rasool N, Tchabo NE, Alejandro EU, Kamrava MM, Kohn EC
    Carcinogenesis, 2007;29(3):466-72.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. First in human phase I trial of 852A, a novel systemic toll-like receptor 7 agonist, to activate innate immune responses in patients with advanced cancer.
    Authors: Dudek AZ, Yunis C, Harrison LI, Kumar S, Hawkinson R, Cooley S, Vasilakos JP, Gorski KS, Miller JS
    Clin. Cancer Res., 2007;13(23):7119-25.
    Species: Human
    Sample Types: Serum
  79. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis.
    Authors: Lepej SZ, Misic-Majerus L, Jeren T, Rode OD, Remenar A, Sporec V, Vince A
    Acta Neurol. Scand., 2007;115(2):109-14.
  80. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
    Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
    Stem Cells, 2007;26(1):279-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
    Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
    Chest, 2006;130(4):982-8.
    Species: Human
    Sample Types: BALF
  82. Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: relevance for the development of human breast cancer.
    Authors: Datta D, Flaxenburg JA, Laxmanan S, Geehan C, Grimm M, Waaga-Gasser AM, Briscoe DM, Pal S
    Cancer Res., 2006;66(19):9509-18.
    Species: Human
    Sample Types: Cell Culture Supernates
  83. Induction of dendritic cell maturation by pertussis toxin and its B subunit differentially initiate Toll-like receptor 4-dependent signal transduction pathways.
    Authors: Wang ZY, Yang D, Chen Q, Leifer CA, Segal DM, Su SB, Caspi RR, Howard ZO, Oppenheim JJ
    Exp. Hematol., 2006;34(8):1115-24.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function.
    Authors: Skoberne M, Somersan S, Almodovar W, Truong T, Petrova K, Henson PM, Bhardwaj N
    Blood, 2006;108(3):947-55.
    Species: Human
    Sample Types: Cell Culture Supernates
  85. Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
    Authors: Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B
    J. Neuroimmunol., 2005;163(1):128-34.
    Species: Human
    Sample Types: Serum
  86. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  87. Deoxycytidyl-deoxyguanosine oligonucleotide classes A, B, and C induce distinct cytokine gene expression patterns in rhesus monkey peripheral blood mononuclear cells and distinct alpha interferon responses in TLR9-expressing rhesus monkey plasmacytoid dendritic cells.
    Authors: Abel K, Wang Y, Fritts L, Sanchez E, Chung E, Fitzgerald-Bocarsly P, Krieg AM, Miller CJ
    Clin. Diagn. Lab. Immunol., 2005;12(5):606-21.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
  88. Simian human immunodeficiency virus-associated pneumonia correlates with increased expression of MCP-1, CXCL10, and viral RNA in the lungs of rhesus macaques.
    Authors: Sui Y, Li S, Pinson D, Adany I, Li Z, Villinger F, Narayan O, Buch S
    Am. J. Pathol., 2005;166(2):355-65.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Tissue Homogenates
  89. CpG oligonucleotides induce strong humoral but only weak CD4+ T cell responses to protein antigens in rhesus macaques in vivo.
    Authors: Hartmann G, Marschner A, Viveros PR, Stahl-Hennig C, Eisenblatter M, Suh YS, Endres S, Tenner-Racz K, Uberla K, Racz P, Steinman RM, Ignatius R
    Vaccine, 2005;23(25):3310-7.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  90. Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
    Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
    Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24.
    Species: Human
    Sample Types: BALF
  91. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
    Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
    Transplantation, 2004;78(9):1341-50.
    Species: Human
    Sample Types: Urine
  92. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus.
    Authors: Wald O, Pappo O, Safadi R, Dagan-Berger M, Beider K, Wald H, Franitza S, Weiss I, Avniel S, Boaz P, Hanna J, Zamir G, Eid A, Mandelboim O, Spengler U, Galun E, Peled A
    Eur. J. Immunol., 2004;34(4):1164-74.
    Species: Human
    Sample Types: Plasma
  93. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003;73(5):584-90.
    Species: Human
    Sample Types: CSF
  94. Helper T-lymphocyte-related chemokines in healthy newborns.
    Authors: Leung TF, Ng PC, Tam WH, Li CY, Wong E, Ma TP, Lam CW, Fok TF
    Pediatr. Res., 2003;55(2):334-8.
    Species: Human
    Sample Types: Serum
  95. TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
    Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
    Eur. J. Immunol., 2002;32(7):2037-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  96. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human CXCL10/IP-10 Quantikine ELISA Kit
By Anonymous on 09/10/2018
Sample Tested: 4T1 mouse breast cancer cell line

I used for the mouse samples since it had 60% similarity with human but didnot detect any protein of interest. However, the exoeriment well ok with nice standard curve


Human CXCL10/IP-10 Quantikine ELISA Kit
By Anonymous on 04/24/2018
Sample Tested: A172 human glioblastoma cell line

Human CXCL10/IP-10 Quantikine ELISA Kit
By Anonymous on 09/08/2017
Sample Tested: Serum

Human CXCL10/IP-10 Quantikine ELISA Kit
By Anonymous on 04/22/2017
Sample Tested: Serum and Plasma