CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
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Human CXCL10/IP-10 Quantikine ELISA Kit
R&D Systems | Catalog # DIP100
Quantikine Quickit version available - results in 90 minutes (QK266).
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (75 µL), EDTA Plasma (75 µL), Heparin Plasma (75 µL), Saliva (10 µL), Urine (100 µL)
Sensitivity
4.46 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
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Product Summary for Human CXCL10/IP-10 Quantikine ELISA Kit
The Quantikine Human IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IP-10 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IP-10 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IP-10.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human IP-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for
the presence of human IP-10 in this assay. No medical histories were available for the donors
used in this study.
ND=Non-detectable
THP-1 human acute monocyte leukemia cells were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were stimulated with 1.0 μg/mL recombinant human IFN-gamma for 8 hours and then 1.0 μg/mL LPS was added. Cells were incubated for an additional 18 hours. An aliquot of the cell culture supernate was removed, diluted 600-fold and assayed, and measured 164,640 pg/mL.
| Sample Type | Mean (pg/mL) | Range (pg/mL) |
| Serum (n=60) | 89 | 38-361 |
| EDTA plasma (n=35) | 96 | 47-382 |
| Heparin plasma (n=35) | 110 | 52-450 |
| Saliva (n=5) | 729 | 292-1340 |
| Sample Type | Mean of Detectable (pg/mL) | % Detectable | Range (pg/mL) |
| Urine (n=18) | 17.2 | 67 | ND-49.7 |
Cell Culture Supernates:
Human peripheral blood cells (1 x 106
cells/mL) were cultured in DMEM supplemented with
5% fetal bovine serum, 50 mM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and
100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with
10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of
human IP-10.
| Condition | Day 1 (pg/mL) | Day 5 (pg/mL) |
| Unstimulated | 29,774 | 21,900 |
| Stimulated | 18,456 | 11,091 |
THP-1 human acute monocyte leukemia cells were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were stimulated with 1.0 μg/mL recombinant human IFN-gamma for 8 hours and then 1.0 μg/mL LPS was added. Cells were incubated for an additional 18 hours. An aliquot of the cell culture supernate was removed, diluted 600-fold and assayed, and measured 164,640 pg/mL.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 70.3 | 174 | 342 | 80.7 | 194 | 362 |
| Standard Deviation | 2.07 | 5.15 | 10.74 | 7.91 | 13.48 | 24.27 |
| CV% | 2.9 | 3.0 | 3.1 | 9.8 | 6.9 | 6.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 67.4 | 168 | 334 | 70.7 | 182 | 366 |
| Standard Deviation | 3.12 | 4.99 | 11.15 | 6.23 | 11.12 | 18.93 |
| CV% | 4.6 | 3.0 | 3.3 | 8.8 | 6.1 | 5.2 |
Recovery for Human CXCL10/IP-10 Quantikine ELISA Kit
The recovery of IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 88-101 |
| EDTA Plasma (n=5) | 99 | 90-109 |
| Heparin Plasma (n=5) | 99 | 87-113 |
| Serum (n=5) | 99 | 88-112 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IP-10 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human CXCL10/IP-10 Quantikine ELISA Kit
Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD5K Standard Curve
Human CXCL10/IP-10/CRG-2 ELISA Calibrator Diluent RD6Q Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL10/IP-10/CRG-2
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Human CXCL10/IP-10 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CXCL10/IP-10 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human CXCL10/IP-10 Quantikine ELISA Kit
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12 Customer Ratings
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Sample Tested: Plasma and UrineVerified Customer | Posted 11/07/2022Can detect in human plasma at 10-fold and in human urine at 5-fold.
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Sample Tested: SerumVerified Customer | Posted 08/14/2022
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Sample Tested: Cell culture supernatantVerified Customer | Posted 03/28/2022
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Sample Tested: SerumVerified Customer | Posted 02/25/2022
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Sample Tested: SerumVerified Customer | Posted 01/14/2022
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Sample Tested: SerumVerified Customer | Posted 09/29/2021
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Sample Tested: SerumVerified Customer | Posted 10/04/2020IP-10 Quantikine assay works better than in the Luminex panel
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Sample Tested: 4T1 mouse breast cancer cell lineVerified Customer | Posted 09/10/2018I used for the mouse samples since it had 60% similarity with human but didnot detect any protein of interest. However, the exoeriment well ok with nice standard curve
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Sample Tested: A375 human melanoma cell lineVerified Customer | Posted 05/17/2018
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Sample Tested: A172 human glioblastoma cell lineVerified Customer | Posted 04/24/2018
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Sample Tested: SerumVerified Customer | Posted 09/08/2017
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Sample Tested: Serum and PlasmaVerified Customer | Posted 04/22/2017
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Protocols
View specific protocols for Human CXCL10/IP-10 Quantikine ELISA Kit (DIP100):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- For Cell Culture Supernate, Saliva, & Urine Samples: Add 150 µL of Assay Diluent to each well.
For Serum & Plasma Samples: Add 75 µL of Assay Diluent to each well. - For Cell Culture Supernate, Saliva, & Urine Samples: Add 100 µL of Standard, control, or sample to each well.
For Serum & Plasma Samples: Add 75 µL of Standard, control, or sample to each well. - Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Assay Diluent
Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
