Detects human CXCL13/BLC/BCA-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL‑8, rhCXCL10, rhCXCL9, rhCXCL12/SDF‑1 alpha, or rhCXCL12/SDF‑1 beta.
Monoclonal Mouse IgG1 Clone # 53610
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CXCL13/BLC/BCA-1 Val23-Arg94 Accession # O43927
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700
Intracellular Staining by Flow Cytometry
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CXCL13/BLC/BCA‑1 in Human Immature Dendritic Cells by Flow Cytometry. Human immature dendritic cells (CD14+ PBMC cultured with 20 ng/mL rhIL-4 and 50 ng/mL rhGM-CSF for 7 days) were stained with Mouse Anti-Human CXCL13/BLC/BCA‑1 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # IC801N, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/ Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
Gunn, M.D. et al. (1998) Nature, 391:799.
Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
Forster, R. et al. (1996) Cell 87:1037.
Entrez Gene IDs:
10563 (Human); 55985 (Mouse)
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
1 Citations: Showing 1 - 1
Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis Authors: DA Rao, MF Gurish, JL Marshall, K Slowikowsk, CY Fonseka, Y Liu, LT Donlin, LA Henderson, K Wei, F Mizoguchi, NC Teslovich, ME Weinblatt, EM Massarotti, JS Coblyn, SM Helfgott, YC Lee, DJ Todd, VP Bykerk, SM Goodman, AB Pernis, LB Ivashkiv, EW Karlson, PA Nigrovic, A Filer, CD Buckley, JA Lederer, S Raychaudhu, MB Brenner Nature, 2017;542(7639):110-114. Species: Human Sample Type: Whole Cells Application: Flow
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