Human CXCL4/PF4 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
IC7952F
Detection of CXCL4/PF4 in Human Immature Dendritic Cells by Flow Cytometry.
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Human CXCL4/PF4 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL4/PF4 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) CXCL6, recombinant human (rh) CXCL6, rhCXCL7, rmCXCL13 or rhCCL21 is observed.
Source
Monoclonal Mouse IgG2B Clone # 170138
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL4/PF4
Glu32-Ser101
Accession # P02776.2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of CXCL4/PF4 in Human Immature Dendritic Cells by Flow Cytometry. View Larger

Detection of CXCL4/PF4 in Human Immature Dendritic Cells by Flow Cytometry. Human immature dendritic cells were stained with Mouse Anti-Human CXCL4/PF4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC7952F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL4/PF4

CXCL4, also known as Platelet Factor 4 (PF4), is an 8 kDa CXC chemokine that is stored in platelet alpha -granules as a homotetramer and secreted abundantly during platelet activation. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein that includes granule targeting and heparin-binding sequences. CXCL4 has homology with IL-8 and beta -thromboglobulin and can form heteromultimers with IL-8. Mature human and mouse CXCL4 share 76% aa identity. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, has anti-angiogenic activity and promotes granule Protein C activation. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains.

References
  1. Poncz, M. et al. (1987) Blood 69:219.
  2. Scheuerer, B. et al. (2000) Blood 95:1158.
  3. Perollet, C. et al. (1998) Blood 91:3289.
  4. Petersen, F. et al. (1998) J. Immunol. 161:4347.
  5. Petersen, F. et al. (1999) J. Biol. Chem. 274:12376.
  6. Watanabe, O. et al. (1999) J. Hum. Genet. 44:173.
Entrez Gene IDs
5196 (Human); 56744 (Mouse); 360918 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; CXCL4; CXCL4iroplact; Iroplact; MGC138298; Oncostatin-A; PF4; PF-4; platelet factor 4; SCYB4oncostatin-A

Product Datasheets

Citation for Human CXCL4/PF4 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The effect of human placental chorionic villi derived mesenchymal stem cell on triple-negative breast cancer hallmarks
    Authors: AT Alshareeda, E Rakha, A Alghwainem, B Alrfaei, B Alsowayan, A Albugami, AM Alsubayyil, M Abomraee, NK Mohd Zin
    PLoS ONE, 2018;13(11):e0207593.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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