Human CXCL8/IL-8 QuantiGlo ELISA Kit

(8 citations)   
  • Assay Length
    5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
  • Sensitivity
    0.97 pg/mL
  • Assay Range
    1.6 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human IL-8
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The QuantiGlo Human IL-8 Chemiluminescent Immunoassay is a 5 hour solid phase ELISA designed to measure human IL-8 levels in cell culture supernates, serum, and plasma. It contains the 72 amino acid variant of human IL-8 derived from E. coli as well as antibodies raised against this recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-8. Results obtained using natural human IL-8 showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-8.

Intra-Assay Precision (Precision within an assay) Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Four samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.2411.028.981460.4811.5714.7216


The recovery of IL-8 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 98-107
Citrate Plasma (n=4) 101 97-107
EDTA Plasma (n=4) 105 95-113
Heparin Plasma (n=4) 107 102-113
Serum (n=4) 103 93-107
To assess the linearity of the assay, samples spiked with high concentrations of IL-8 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL8/IL-8 QuantiGlo ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL8/IL-8
CXCL8/Interleukin-8 is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. CXCL8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. CXCL8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents CXCL8 interaction with CXCR1.
    • Entrez Gene IDs
      3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline);
    • Alternate Names
      3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1;
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. Activation of nuclear factor-kappa B signalling promotes cellular senescence.
      Authors: Rovillain E, Mansfield L, Caetano C, Alvarez-Fernandez M, Caballero OL, Medema RH, Hummerich H, Jat PS
      Oncogene, 2011;30(20):2356-66.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
      Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
      Inflamm. Res., 2010;59(5):339-47.
      Species: Human
      Sample Type: Serum
    3. Presence of cytokeratins in exhaled breath condensate of mechanical ventilated patients.
      Authors: Gessner C, Dihazi H, Brettschneider S, Hammerschmidt S, Kuhn H, Eschrich K, Keller T, Engelmann L, Sack U, Wirtz H
      Respir Med, 2007;102(2):299-306.
      Species: Human
      Sample Type: EBC
    4. Phase I clinical and pharmacodynamic evaluation of oral CI-1033 in patients with refractory cancer.
      Authors: Zinner RG, Nemunaitis J, Eiseman I, Shin HJ, Olson SC, Christensen J, Huang X, Lenehan PF, Donato NJ, Shin DM
      Clin. Cancer Res., 2007;13(10):3006-14.
      Species: Human
      Sample Type: Plasma
    5. Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells.
      Authors: Fichorova RN, Trifonova RT, Gilbert RO, Costello CE, Hayes GR, Lucas JJ, Singh BN
      Infect. Immun., 2006;74(10):5773-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Recombinant human antithrombin inhibits thrombin formation and interleukin 6 release in human endotoxemia.
      Authors: Leitner JM, Firbas C, Mayr FB, Reiter RA, Steinlechner B, Jilma B
      Clin. Pharmacol. Ther., 2006;79(1):23-34.
      Species: Human
      Sample Type: Plasma
    7. Endurance training reduces circulating inflammatory markers in persons at risk of coronary events: impact on plaque stabilization?
      Authors: Niessner A, Richter B, Penka M, Steiner S, Strasser B, Ziegler S, Heeb-Elze E, Zorn G, Leitner-Heinschink A, Niessner C, Wojta J, Huber K
      Atherosclerosis, 2005;186(1):160-5.
      Species: Human
      Sample Type: Plasma
    8. Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study.
      Authors: Watt G, Kantipong P, Jongsakul K, de Souza M, Burnouf T
      QJM, 2001;94(11):599-607.
      Species: Human
      Sample Type: Plasma
    ELISA Controls
    Description Application Cat# Citations Images  

    QuantiGlo Immunoassay Control Set 732 for Human CXCL8/IL-8

    Ctrl QC194
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