Human CXCR7/RDC-1 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human CXCR7/RDC‑1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of clone 358426 may result in an underestimation of CXCR7 expression on certain cell types.
  • Source
    Monoclonal Mouse IgG2A Clone # 358426
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CXCR7/RDC‑1
    Met1-Lys362 (Gly131Ser)
    Accession # AAA62370
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR7/RDC‑1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/RDC‑1 APC‑conjugated Mono­clonal Antibody (Catalog # FAB42271A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR7/RDC-1

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti‑CXCR7, clone 9C4) (1, 2) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti‑CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (3). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

  • References:
    1. Balabanian, K. et al. (2005) J. Biol. Chem. 280:35760.
    2. Infantino, S. et al. (2006) J. Immunol. 176:2197.
    3. Burns, J.M. et al. (2006) J. Exp. Med. 203:2201.
  • Entrez Gene IDs:
    57007 (Human); 12778 (Mouse); 84348 (Rat)
  • Alternate Names:
    ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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