Human Cystatin D Alexa Fluor® 700-conjugated Antibody

Catalog #: AF1202N Datasheet / COA / SDS

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AF1202N-100UG

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All Cystatin D Antibodies

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Human Cystatin D Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Cystatin C, rhCystatin SN, and rhCystatin S is observed and less than 1% cr

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin D
Thr29-Val142
Accession # CAA49838

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cystatin D

Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).

Entrez Gene IDs

1473 (Human)

Alternate Names

CST5; CSTD; cystatin 5; Cystatin D; Cystatin-5; cystatin-D; cysteine-proteinase inhibitor; MGC71922