Human Cystatin D Antibody

Catalog # Availability Size / Price Qty
Product Details
Supplemental Products

Human Cystatin D Antibody Summary

Species Reactivity
Detects human Cystatin D in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Cystatin C, rhCystatin SN, and rhCystatin S is observed and less than 1% cross-reactivity with rhCystatin E/M and rhFetuin A is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Cystatin D
Accession # CAA49838
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Cystatin D (Catalog # 1202-PI)
Measured by its ability to neutralize Recombinant Human Cystatin D (1 µg/mL, Catalog # 1202-PI) inhibition of Papain (0.1 µg/mL) cleavage of the fluorogenic peptide substrate Z-FR-Amc(100 µM, Catalog # ES009). The Neutralization Dose (ND50) is typically 13 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin D

Cystatin D is a member of family 2 of the cystatin superfamily (1). In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S (2). The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.12‑1.9 μM) and has been suggested to play a protective role against proteases present in the oral cavity (3).

  1. Freije, J.P. et al. (1993) J. Biol. Chem. 268:15737.
  2. Balbin, M. et al. (1994) J. Biol. Chem. 269:23156.
  3. Collins, A.R. and A. Grubb (1998) Oral Microbiol. Immunol. 13:59.
Entrez Gene IDs
1473 (Human)
Alternate Names
CST5; CSTD; cystatin 5; Cystatin D; Cystatin-5; cystatin-D; cysteine-proteinase inhibitor; MGC71922

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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