Human Cytosolic Sulfotransferase 1E1/SULT1E1 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF5545R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5545R-100UG

All Cytosolic Sulfotransferase 1E1/SULT1E1 Antibodies

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Human Cytosolic Sulfotransferase 1E1/SULT1E1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) Sulfotransferase 1A1 is observed and less than 2% cross‑reactivity with rhS

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Sulfotransferase 1E1/SULT1E1
Asn2-Ile294
Accession # P49888

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Cytosolic Sulfotransferase 1E1/SULT1E1

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).

Entrez Gene IDs

6783 (Human); 20860 (Mouse); 25355 (Rat)

Alternate Names

Cytosolic Sulfotransferase 1E1; EC 2.8.2; EST-1; ESTMGC34459; estrone sulfotransferase; ST1E1; STE; STEestrogen sulfotransferase; Sulfotransferase 1E1; sulfotransferase family 1E, estrogen-preferring, member 1; Sulfotransferase, estrogen-preferringEC 2.8.2.4; SULT1E1