Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) Sulfotransferase 1A1 is observed and less than 2% cross‑reactivity with rhSulfotransferase 2A1 and rhSulfotransferase 4A1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Sulfotransferase 1E1/SULT1E1
    Asn2-Ile294
    Accession # P49888
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot.

Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 37kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic Sulfotransferase 1E1/SULT1E1
Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).
  • References:
    1. Gong, H. et al. (2008) Cancer Res. 68:7386.
    2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
    3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
    4. Takase, Y. et al. (2007) The Prostate 67:405.
    5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
    6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
    7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
    8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
     
         
      1. Entrez Gene IDs:
        6783 (Human); 20860 (Mouse); 25355 (Rat)
      2. Alternate Names:
        Cytosolic Sulfotransferase 1E1; EC 2.8.2; EST-1; ESTMGC34459; estrone sulfotransferase; ST1E1; STE; STEestrogen sulfotransferase; Sulfotransferase 1E1; sulfotransferase family 1E, estrogen-preferring, member 1; Sulfotransferase, estrogen-preferringEC 2.8.2.4; SULT1E1
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      Average Rating: 4 (Based on 1 review)

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      Western Blot Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody AF5545
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       WB Anonymous 06/01/2016
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      Western Blot Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody AF5545
      Western Blot: Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody [AF5545]

      Summary

      ApplicationWestern Blot
      Sample TestedLiver tissue,Kidney tissue

      Other Experimental Details

      Other Experimental DetailsPrimary ab dilution 1:2000 (Maybe working with 1:4000)40 ug protein per lane

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