Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

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Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot.
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Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody Summary

Species Reactivity
Detects human Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) Sulfotransferase 1A1 is observed and less than 2% cross‑reactivity with rhSulfotransferase 2A1 and rhSulfotransferase 4A1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human Sulfotransferase 1E1/SULT1E1
Accession # P49888
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
25 µg/mL
Cell lysates spiked with Recombinant Human Cytosolic Sulfotransferase 1E1/SULT1E1 (Catalog # 5545-ST), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 antibody by Western Blot. View Larger

Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 37kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosolic Sulfotransferase 1E1/SULT1E1

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).

  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
      Entrez Gene IDs
      6783 (Human); 20860 (Mouse); 25355 (Rat)
      Alternate Names
      Cytosolic Sulfotransferase 1E1; EC 2.8.2; EST-1; ESTMGC34459; estrone sulfotransferase; ST1E1; STE; STEestrogen sulfotransferase; Sulfotransferase 1E1; sulfotransferase family 1E, estrogen-preferring, member 1; Sulfotransferase, estrogen-preferringEC; SULT1E1

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      Citation for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody

      R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

      1 Citation: Showing 1 - 1

      1. Phiclust: a clusterability measure for single-cell transcriptomics reveals phenotypic subpopulations
        Authors: M Mircea, M Hochane, X Fan, SM Chuva de S, D Garlaschel, S Semrau
        Genome Biology, 2022;23(1):18.
        Species: Human
        Sample Types: Whole Tissue
        Applications: IHC


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      Isotype Controls

      Reconstitution Buffers

      Secondary Antibodies

      Goat IgG (H+L) Antibody


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      Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody
      By Hua Jiang on 06/01/2016
      Application: WB Sample Tested: Kidney tissue,Liver tissue Species: Human and Mouse

      Primary ab dilution 1:2000 (Maybe working with 1:4000)
      40 ug protein per lane