Human DDR2 Alexa Fluor® 594-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human DDR2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 290804
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human DDR2
    Gln24-Arg399
    Accession # Q16832
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human DDR2 in HEK293 Human Cell Line. HEK293 human embryonic kidney cell line transfected with human DDR2 (filled histogram) or irrelevant transfectants (open histogram) were stained with Mouse Anti-Human DDR2 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB25381T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3‑5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10‑12).

  • References:
    1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
    2. Karn, T. et al. (1993) Oncogene 8:3433.
    3. Vogel, W. et al. (1997) Mol. Cell 1:13.
    4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
    5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
    6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
    7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
    8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
    9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
    10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
    11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
    12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.
  • Long Name:
    Discoidin Domain Receptor 2
  • Entrez Gene IDs:
    4921 (Human); 18214 (Mouse)
  • Alternate Names:
    CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase
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Isotype Controls
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Mouse IgG2B Alexa Fluor® 594-conjugated Isotype Control

Ctrl IC0041T
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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