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Human DGK-beta Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB6727N Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB6727N-100UG

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Human DGK-beta Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DGK-beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DGK-alpha, gamma, epsilon, iota, kappa, theta, or zeta is observed.
Source
Monoclonal Mouse IgG1 Clone # 646523
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human DGK-β
Met1-Ser143
Accession # Q9Y6T7
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DGK-beta

Diacylglycerol kinase beta (DGK-beta ) is an approximately 90 kDa kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-beta promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa 149-184 and 194-229), two zinc finger regions (aa 244-294 and aa 309-358), and a catalytic domain (aa 434-568). Alternate splicing generates an additional isoform with a 31 aa C-terminal deletion. Over aa 1-143, human DGK-beta shares 97% aa sequence identity with mouse and rat DGK-beta.

Long Name
Diacylglycerol Kinase, beta 90kDa
Entrez Gene IDs
1607 (Human); 217480 (Mouse); 54248 (Rat)
Alternate Names
90 kDa diacylglycerol kinase; DAG kinase beta; DAGK2; DGKB; DGKbeta; DGK-beta; diacylglycerol kinase beta; diacylglycerol kinase, beta (90kD); diacylglycerol kinase, beta 90kDa; Diglyceride kinase beta; EC 2.7.1; EC 2.7.1.107; KIAA0718DGKDGK-BETA

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