Human DGK-beta Antibody

Catalog # Availability Size / Price Qty
Detection of Human DGK‑ beta  by Western Blot.
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Product Details
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Human DGK-beta Antibody Summary

Species Reactivity
Detects human DGK-beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DGK-alpha, gamma, epsilon, iota, kappa, theta, or zeta is observed.
Monoclonal Mouse IgG1 Clone # 646523
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human DGK-beta
Accession # Q9Y6T7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DGK-beta  antibody by Western Blot. View Larger

Detection of Human DGK‑ beta by Western Blot. Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DGK-beta Monoclonal Antibody (Catalog # MAB6727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-beta at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-beta

Diacylglycerol kinase beta (DGK-beta ) is an approximately 90 kDa kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-beta promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa 149-184 and 194-229), two zinc finger regions (aa 244-294 and aa 309-358), and a catalytic domain (aa 434-568). Alternate splicing generates an additional isoform with a 31 aa C-terminal deletion. Over aa 1-143, human DGK-beta shares 97% aa sequence identity with mouse and rat DGK-beta.

Long Name
Diacylglycerol Kinase, beta 90kDa
Entrez Gene IDs
1607 (Human); 217480 (Mouse); 54248 (Rat)
Alternate Names
90 kDa diacylglycerol kinase; DAG kinase beta; DAGK2; DGKB; DGKbeta; DGK-beta; diacylglycerol kinase beta; diacylglycerol kinase, beta (90kD); diacylglycerol kinase, beta 90kDa; Diglyceride kinase beta; EC 2.7.1; EC; KIAA0718DGKDGK-BETA

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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