Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 646523
View all DGK-beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DGK-beta
Met1-Ser143
Accession # Q9Y6T7

Specificity

Detects human DGK-beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human DGK-alpha, gamma, epsilon, iota, kappa, theta, or zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human DGK‑ beta Antibody

Detection of Human DGK-beta antibody by Western Blot.

Detection of Human DGK‑ beta by Western Blot.

Western blot shows lysates of U-87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DGK-beta Monoclonal Antibody (Catalog # MAB6727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-beta at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Applications for Human DGK‑ beta Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: U‑87 MG human glioblastoma/astrocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DGK-beta

Diacylglycerol kinase beta (DGK-beta ) is an approximately 90 kDa kinase that phosphorylates the lipid diacylglycerol. It is expressed in the brain and colocalizes with D1 and D2 dopamine receptors. DGK-beta promotes the formation and maturation of dendritic spines. It contains two EF-hand motifs (aa 149-184 and 194-229), two zinc finger regions (aa 244-294 and aa 309-358), and a catalytic domain (aa 434-568). Alternate splicing generates an additional isoform with a 31 aa C-terminal deletion. Over aa 1-143, human DGK-beta shares 97% aa sequence identity with mouse and rat DGK-beta.

Long Name

Diacylglycerol Kinase, beta 90kDa

Alternate Names

DAGK2, DGKB, DGKbeta

Entrez Gene IDs

1607 (Human); 217480 (Mouse); 54248 (Rat)

Gene Symbol

DGKB

UniProt

Q9Y6T7

Additional DGK-beta Products

Product Documents for Human DGK‑ beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DGK‑ beta Antibody

For research use only

Related Research Areas

Intracellular Kinases

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