Human DGK-zeta Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: DGK-zeta

DGK-zeta (Diacylglycerol kinase zeta; also DAG kinase 6) is a 130 kDa member of the eukaryotic diacylglycerol (DAG) kinase family of proteins. It exists in both cytoplasm and nucleus, and is widely expressed, being found in neurons, skeletal muscle cells and endothelium. DGK-zeta converts DAG into phosphatidic acid (PA). In the nucleus, this activates mTOR and promotes cell proliferation. In the cytoplasm, DGK-zeta -generated PA activates PAK1, leading to increased cell motility. Human DGK-zeta is 1117 amino acids (aa) in length and contains two DAG-type Zn-finger regions (aa 287‑419), a MARCKS homology domain (aa 448‑462) and a catalytic domain (aa 486‑607). In addition to the 130 kDa protein, there are several splice variants that run about 114 kDa in SDS-PAGE.

Product Datasheets

Related Research Areas

• Intracellular Kinases
• Intracellular Kinases in the Akt Pathway
• Platelet Signaling
• Search Kinases and Phosphatases