Human DLL3 PE-conjugated Antibody

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Detection of DLL3 in U‑118‑MG Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Reviews (1)

Human DLL3 PE-conjugated Antibody Summary

Species Reactivity
Detects human DLL3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) DLL1 is observed and less than 8% cross-reactivity with rhDLL4 is observed.
Polyclonal Goat IgG
Protein A or G purified
Chinese hamster ovary cell line CHO-derived recombinant human DLL3
Accession # Q9NYJ7
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DLL3 antibody in U-118-MG Human Cell Line antibody by Flow Cytometry. View Larger

Detection of DLL3 in U‑118‑MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/astrocytoma cell line was stained with Goat Anti-Human DLL3 PE-conjugated Polyclonal Antibody (Catalog # FAB4315P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DLL3

DLL3 (Delta-like protein 3; also Delta3) is a 65‑67 kDa member of the Delta family of proteins. It is expressed in/on select cell types in the embryo, including somitic mesoderm, cochlear hair cells and noncycling cells of the neural tube that are undergoing differentiation. DLL3 is principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation. Mature human DLL3 is a 592 amino acid (aa) type I transmembrane protein. It possesses a 466 aa extracellular domain (aa 27-492) that contains a Notch-binding DSL domain (aa 176-215) followed by six EGF-like repeats (aa 216-465). There is one isoform variant that contains an Ala substitution for aa 588-618. Over aa 27-488, human DLL3 shares 84% aa identity with mouse DLL3.

Long Name
Delta-like 3
Entrez Gene IDs
10683 (Human); 13389 (Mouse); 114125 (Rat)
Alternate Names
delta (Drosophila)-like 3; Delta3; delta-like 3 (Drosophila); delta-like protein 3; DLL3; Drosophila Delta homolog 3; Pudgy; SCDO1; SCDO1delta3

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Citations for Human DLL3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SCLC-J1, a novel small cell lung cancer cell line
    Authors: K Ohara, S Kinoshita, J Ando, Y Azusawa, M Ishii, S Harada, Y Mitsuishi, T Asao, K Tajima, T Yamamoto, F Takahashi, N Komatsu, K Takahashi, M Ando
    Biochemistry and Biophysics Reports, 2021-07-28;27(0):101089.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria
    Authors: H Yu, W Hu, X Song, D Descalzi-M, Z Yang, R Korngold, Y Zhao
    Int J Mol Sci, 2020-06-15;21(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human DLL3 PE-conjugated Antibody
By Anonymous on 11/01/2021
Application: Flow Sample Tested: Cancer Tissue Species: Human

Detection of DLL3 on CD45- cells from a Non-Small Cell Lung Cancer patient tissue. Single-cell suspension was generated from a lung cancer tissue biopsy and cells were treated a CD45 antibody along with the goat polyclonal Human DLL3 PE-conjugated Antibody (catalog # FAB4315P) (BLUE) or Goat IgG PE-conjugated Antibody (catalog # IC108P) (RED).