DLL3 (Delta-like protein 3; also Delta3) is a 65‑67 kDa member of the Delta family of proteins. It is expressed in/on select cell types in the embryo, including somitic mesoderm, cochlear hair cells and noncycling cells of the neural tube that are undergoing differentiation. DLL3 is principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation. Mature human DLL3 is a 592 amino acid (aa) type I transmembrane protein. It possesses a 466 aa extracellular domain (aa 27-492) that contains a Notch-binding DSL domain (aa 176-215) followed by six EGF-like repeats (aa 216-465). There is one isoform variant that contains an Ala substitution for aa 588-618. Over aa 27-488, human DLL3 shares 84% aa identity with mouse DLL3.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala27-Pro488
Accession # Q9NYJ7
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human DLL3 Antibody
Detection of Human DLL3 by Western Blot.
Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DLL3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of DLL3 in U‑118‑MG Human Cell Line by Flow Cytometry.
U-118-MG human glioblastoma/astrocytoma cell line was stained with Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Applications for Human DLL3 Antibody
Flow Cytometry
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line
Western Blot
Sample: Hs 294T human melanoma cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: DLL3
Long Name
Alternate Names
Gene Symbol
UniProt
Additional DLL3 Products
Product Documents for Human DLL3 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human DLL3 Antibody
For research use only
Related Research Areas
Citations for Human DLL3 Antibody
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Protocols
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Associated Pathways
