Detects human DLL3 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) DLL1 is observed, and less than 8% cross-reactivity with rhDLL4 is observed.
Polyclonal Goat IgG
Protein A or G purified
Chinese hamster ovary cell line CHO-derived recombinant human DLL3 Ala27-Pro488 Accession # Q9NYJ7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DLL3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of DLL3 in U‑118‑MG Human Cell Line by Flow Cytometry.
U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Goat Anti-Human DLL3 Polyclonal Antibody (Catalog # AB4315, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DLL3 (Delta-like protein 3; also Delta3) is a 65‑67 kDa member of the Delta family of proteins. It is expressed in/on select cell types in the embryo, including somitic mesoderm, cochlear hair cells and noncycling cells of the neural tube that are undergoing differentiation. DLL3 is principally found in the Golgi apparatus and endosomes where it promotes Notch family receptor degradation. Mature human DLL3 is a 592 amino acid (aa) type I transmembrane protein. It possesses a 466 aa extracellular domain (aa 27-492) that contains a Notch-binding DSL domain (aa 176-215) followed by six EGF-like repeats (aa 216-465). There is one isoform variant that contains an Ala substitution for aa 588-618. Over aa 27-488, human DLL3 shares 84% aa identity with mouse DLL3.
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