Human DOT1L Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB6546AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 653613
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Product Specifications

Specificity

Detects human DOT1L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PCDH-8, rhNME-3, or rhWISP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human DOT1L Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DOT1L

DOT1L (mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa (predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is involved in developmental gene transcription and maintenance of chromatin structure, and may promote mixed lineage leukemia. Within the sequence used as an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third isoform of 1739 aa has a 202 aa C-terminal extension.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Entrez Gene IDs

84444 (Human)

Gene Symbol

DOT1L

UniProt

Additional DOT1L Products

Product Documents for Human DOT1L Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DOT1L Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

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Protocols

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