Human DOT1L Antibody

Catalog # Availability Size / Price Qty
MAB6546
MAB6546-SP
DOT1L in Human Lung.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Human DOT1L Antibody Summary

Species Reactivity
Human
Specificity
Detects human DOT1L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PCDH-8, rhNME-3, or rhWISP-3 is observed.
Source
Monoclonal Mouse IgG2A Clone # 653613
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DOT1L
Gly2-Lys416
Accession # Q8TEK3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Cell lysates spiked with recombinant human DOT1L, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry DOT1L antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

DOT1L in Human Lung. DOT1L was detected in immersion fixed paraffin-embedded sections of human lung using Human DOT1L Monoclonal Antibody (Catalog # MAB6546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DOT1L

DOT1L (mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa (predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is involved in developmental gene transcription and maintenance of chromatin structure, and may promote mixed lineage leukemia. Within the sequence used as an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third isoform of 1739 aa has a 202 aa C-terminal extension.

Long Name
Disruptor of Telomeric Silencing 1-like
Entrez Gene IDs
84444 (Human)
Alternate Names
DOT1L; DOT1-like protein; DOT1-like, histone H3 methyltransferase (S. cerevisiae); EC 2.1.1.43; H3-K79-HMTase; KIAA1814DKFZp586P1823; KMT4; KMT4H3 lysine-79 specific; Lysine N-methyltransferase 4

Product Datasheets

Citation for Human DOT1L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. DOT1L safeguards cartilage homeostasis and protects against osteoarthritis
    Authors: S Monteagudo, FMF Cornelis, C Aznar-Lope, P Yibmantasi, LA Guns, P Carmeliet, F Cailotto, RJ Lories
    Nat Commun, 2017;8(0):15889.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human DOT1L Antibody

Average Rating: 5 (Based on 1 Review)

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Human DOT1L Antibody
By Anonymous on 05/19/2022
Application: IHC Sample Tested: Lung tissue Species: Human