Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunoprecipitation

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 653613
View all DOT1L Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DOT1L
Gly2-Lys416
Accession # Q8TEK3

Specificity

Detects human DOT1L in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PCDH-8, rhNME-3, or rhWISP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human DOT1L Antibody

DOT1L antibody in Human Lung by Immunohistochemistry (IHC-P).

DOT1L in Human Lung.

DOT1L was detected in immersion fixed paraffin-embedded sections of human lung using Human DOT1L Monoclonal Antibody (Catalog # MAB6546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human DOT1L Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with recombinant human DOT1L, see our available Western blot detection antibodies

Reviewed Applications

Read 1 review rated 5 using MAB6546 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DOT1L

DOT1L (mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa (predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is involved in developmental gene transcription and maintenance of chromatin structure, and may promote mixed lineage leukemia. Within the sequence used as an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third isoform of 1739 aa has a 202 aa C-terminal extension.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Entrez Gene IDs

84444 (Human)

Gene Symbol

DOT1L

UniProt

Q8TEK3

Additional DOT1L Products

Product Documents for Human DOT1L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DOT1L Antibody

For research use only

Related Research Areas

Epigenetics

Citations for Human DOT1L Antibody

Customer Reviews for Human DOT1L Antibody (1)

5 out of 5
1 Customer Rating
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  • Human DOT1L Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 05/19/2022
    Human DOT1L Antibody MAB6546

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Protocols

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FAQs

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