DOT1L in Human Lung.
DOT1L was detected in immersion fixed paraffin-embedded sections of human lung using Human DOT1L Monoclonal Antibody (Catalog # MAB6546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(mammalian counterpart of yeast Dot1) is a 1537 amino acid (aa), 165 kDa
(predicted) nuclear non-SET domain histone H3K79 methyltransferase. It is
involved in developmental gene transcription and maintenance of chromatin
structure, and may promote mixed lineage leukemia. Within the sequence used as
an immunogen, human DOT1L shares 97% and 94% aa identity with mouse and rat
DOT1L, respectively. Two human isoforms affect this region: a 1467 aa form has
an alternate start site at aa 71, while a 1513 aa form lacks aa 89-112. A third
isoform of 1739 aa has a 202 aa C-terminal extension.
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