Human umbilical cord endothelial cells cultured for 4 hours in the presence of 10 ng/mL of rhTNF-a were stained with anti-human CD62E-Fluorescein (R&D Systems, Cat. # BBA21, filled histogram) or with isotype control (R&D Systems, Cat. # IC002F, open histogram).
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E) is a 115 kDa type-I membrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines such as IL-1 beta and TNF-alpha or endotoxin (1 - 4). Expression is transitory reaching a maximum within 6 hours of stimulation and then declining with the generation of a soluble form of E-Selectin (1 - 5). Expression of E-Selectin on cell surfaces facilitates the rolling attachment of leukocytes to the endothelium which is an important step in the extravasation of leukocytes at sites of inflammation (1 - 6). E-Selectin is thought to play a prominent role in inflammatory precesses of the skin (4).
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