Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody

R&D Systems | Catalog # BBA21

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E5
View all E-Selectin/CD62E Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human E‑Selectin/CD62E

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody

Detection of CD62E antibody in HUVECs antibody by Flow Cytometry.

Detection of CD62E in HUVECs by Flow Cytometry.

Human umbilical cord endothelial cells cultured for 4 hours in the presence of 10 ng/mL of rhTNF-a were stained with anti-human CD62E-Fluorescein (R&D Systems, Cat. # BBA21, filled histogram) or with isotype control (R&D Systems, Cat. # IC002F, open histogram).

Applications for Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/mL
Sample: Human umbilical cord endothelial cells

Reviewed Applications

Read 1 review rated 5 using BBA21 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E) is a 115 kDa type-I membrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines such as IL-1 beta and TNF-alpha or endotoxin (1 - 4). Expression is transitory reaching a maximum within 6 hours of stimulation and then declining with the generation of a soluble form of E-Selectin (1 - 5). Expression of E-Selectin on cell surfaces facilitates the rolling attachment of leukocytes to the endothelium which is an important step in the extravasation of leukocytes at sites of inflammation (1 - 6). E-Selectin is thought to play a prominent role in inflammatory precesses of the skin (4).

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody

For research use only

Citations for Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody

Customer Reviews for Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human E‑Selectin/CD62E Fluorescein‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human E-Selectin/CD62E Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood cells
    Species: Human
    Verified Customer | Posted 07/07/2017

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies