Human EGF XL Luminex® Performance Assay

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EGF XL Cytokine Luminex Panel Standard Curves
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Human EGF XL Luminex® Performance Assay Summary

Panel Luminex Performance Human XL Cytokine Panel
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

View Luminex High Performance Human XL validation data.

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Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed
User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

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Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile Human EGF with up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Base Kit Components
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**
  • High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents
  • User-mix antibody-coated Magnetic beads in individual vials

Recommended Sample Types: Cell culture supernates, serum, and platelet-poor plasma

Components: Human EGF Magnetic Microparticles (Part 898847) is supplied as a 100X concentrated stock (0.075 mL) with preservatives.

Other Supplies Required: Magnetic Luminex® Performance Assay Human XL Discovery Base Kit (R&D Systems®, Catalog # LUXLM000).

Sensitivity: The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the mean MFI of twenty zero standard replicates and calculating the corresponding concentration.
Six assays were evaluated, and the MDD of human EGF ranged from 0.743-3.70 pg/mL. The mean MDD was 1.21 pg/mL.

Correlation: This assay has been correlated to the Quantikine® ELISA Kit.

Specificity: This assay recognizes natural and recombinant human EGF.

Bead Region: 33
 

Precision

Intra-assay Precision (precision within an assay) - Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (precision between assays) - Two samples of known concentration were tested in thirty separate assays to assess precision between assays.

  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 30 30
Mean (pg/mL) 73.8 726 77.5 745
Standard deviation 1.78 41.4 8.17 40.9
CV (%) 41.1 70.2 10.5 49.6
 

Recovery

Samples containing and/or spiked with human EGF were evaluated for recovery.

Sample Type Average % Recovery Range (%)
Cell culture supernate 115 107-124
Serum 64.8 50.7-80.5
EDTA plasma 75.2 59.4-97.4
Heparin plasma 90.1 60.3-130
Plaelet-poor EDTA 85.5 69.4-102
Platelet-poor Heparin 88.5 68.5-110
 

Linearity

Samples containing and/or spiked with human EGF were serially diluted to evaluate assay linearity.

  Cell culture supernate Serum EDTA plasma Heparin plasma Platelet-poor EDTA Platelet-poor Heparin
1:2 Average % of Expected 97.6 137 125 123 126 122
Range (%) 93.7-104 134-143 115-132 106-134 121-130 119-125
1:4 Average % of Expected 95.0 148 124 120 130 124  
Range (%) 90.7-100 141-157 111-138 104-139 120-139 118-129
1:8 Average % of Expected 95.1 157 128 123 138 124
Range (%) 91.5-97.3 144-174 108-149 103-144 125-165 111-129
1:16 Average % of Expected 95.1 156 126 120 135 121
Range (%) 92.1-97.9 143-180 106-151 99.3-150 131-142 104-135
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

EGF XL Cytokine Luminex Panel Standard Curves

Background: EGF

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Long Name
Epidermal Growth Factor
Entrez Gene IDs
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citation for Human EGF XL Luminex® Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiple cytokine analyses of aqueous humor from the patients with retinitis pigmentosa
    Authors: B Lu, H Yin, Q Tang, W Wang, C Luo, X Chen, X Zhang, K Lai, J Xu, X Chen, K Yao
    Cytokine, 2019-12-03;127(0):154943.
    Species: Human
    Sample Types: Aqueous Humor

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