Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 908235
View all EMR3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human EMR3
Met1-Tyr652
Accession # NP_115960

Specificity

Stains human EMR3 transfected but not irrelevant transfected cells in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human EMR3 APC‑conjugated Antibody

Detection of EMR3 antibody in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP antibody by Flow Cytometry.

Detection of EMR3 in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human EMR3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.
Detection of EMR3 antibody in Human Peripheral Blood Monocytes antibody by Flow Cytometry.

Detection of EMR3 in Human Peripheral Blood Monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human EMR3 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood monocytes and HEK293 human embryonic kidney cell line transfected with human EMR3 and eGFP

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EMR3

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Long Name

EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3

Alternate Names

egf-like module containing, mucin-like, hormone receptor-like 3, EGF-like module receptor 3, EGF-like module-containing mucin-like hormone receptor-like 3, egf-like module-containing mucin-like receptor 3

Entrez Gene IDs

84658 (Human)

Gene Symbol

ADGRE3

UniProt

NP_115960

Additional EMR3 Products

Product Documents for Human EMR3 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human EMR3 APC‑conjugated Antibody

For research use only

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