Human EMR3 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8496
MAB8496-SP
Detection of EMR3 in Human Blood Monocytes by Flow Cytometry.
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Human EMR3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EMR3 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 908235
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human EMR3
Met1-Tyr652
Accession # Q9BY15
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of EMR3 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of EMR3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human EMR3 Monoclonal Antibody (Catalog # MAB8496) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMR3

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Long Name
EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3
Entrez Gene IDs
84658 (Human)
Alternate Names
egf-like module containing, mucin-like, hormone receptor-like 3; EGF-like module receptor 3; EGF-like module-containing mucin-like hormone receptor-like 3; egf-like module-containing mucin-like receptor 3; EMR3

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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