Human EphA2 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human EphA2 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse (rm) EphA2 is observed and less than 1% cross-reactivity with recombinant human (rh) EphA1, rmEphA4, rhEphA5, rmEphA6, rmEphA7, and rmEphA8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EphA2
    Gln25-Asn534
    Accession # P29317
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human EphA2 (Catalog # 3035-A2)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EphA2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3035, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphA2

EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5 (1, 2, 3, 4). A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains (5). The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM) (5). The ECD of human EphA2 shares 90‑94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding (6, 7) and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways (8, 9). Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin (10), and can be induced by p53 family transcription factors (11). EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells (12, 13). In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels (14). EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration (13). The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors (15).

  • References:
    1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
    2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419. 
    3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
    4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
    5. Bohme, B et al. (1993) Oncogene 8:2857.
    6. Pandey, A. et al. (1995) Science 268:567.
    7. Bartley, T.D. et al. (1994) Nature 368:558.
    8. Pandey, A. et al. (1994) J. Biol. Chem. 269:30154.
    9. Miao, H. et al. (2001) Nat. Cell Biol. 3:527.
    10. Orsulic, S. and R. Kemler (2000) J. Cell Sci. 113:1793.
    11. Dohn, M. et al. (2001) Oncogene 20:6503.
    12. Zelinski, D.P. et al. (2001) Cancer Res. 61:2301.
    13. Brantley, D.M. et al. (2002) Oncogene 21:7011.
    14. Ogawa, K. et al. (2000) Oncogene 19:6043.
    15. Sulman, E.P. et al. (1997) Genomics 40:371.
  • Entrez Gene IDs:
    1969 (Human); 13836 (Mouse)
  • Alternate Names:
    ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK
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