Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu4F2
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Human ErbB2/Her2

Specificity

Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

ErbB2/Her2 in SK‑BR‑3 Human Cell Line.

ErbB2/Her2 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line (positive staining) and THP‑1 human acute monocytic leukemia cell line (negative staining) using Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB11042) at 5 µg/mL for 3 hours at room temperature. Cells were stained using Anti-human-Cy3 antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed SK‑BR‑3 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB2). This product constitutes the immunogen used for the anti-Idiotype catalog numbers MAB9547 and MAB95471. The biotinylated antibody (catalog number FAB9589B) can be used in flow cytometry or various assay formats to measure Her2 expression and can be detected by conjugated streptavidin.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

For research use only

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