Human Erythropoietin/EPO Quantikine ELISA Kit, RUO New

Catalog #: DEPRU0B Datasheet / COA / SDS

Human Erythropoietin/EPO Quantikine ELISA Kit, RUO

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Human Erythropoietin Quantikine ELISA Standard Curve

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Erythropoietin/EPO Quantikine ELISA Kit, RUO Summary

Assay Length

2.5 hours

Specificity

Natural and recombinant human Epo.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Epo Immunoassay is a 2.5 hour solid phase immunoassay designed to measure human Epo in serum and EDTA plasma. It contains recombinant human Epo, and antibodies raised against the recombinant protein. Natural human Epo showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human Epo.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess Intra-Assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess Inter-Assay Precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (mIU/mL)	10.3	40.3	117	10.4	41.9	118
Standard Deviation	0.37	1.45	1.97	0.86	2.2	5.78
CV%	3.6	3.6	1.7	8.3	5.3	4.9

Recovery

The recovery of human Epo spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	84-98
Serum (n=4)	101	96-107

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human Epo in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human Erythropoietin Quantikine ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin/EPO

Erythropoietin (Epo) is a 30-kDa, heavily glycosylated protein produced primarily by the kidney. It is the principal factor that regulates erythropoiesis. The production of Epo by kidney cells is increased in response to hypoxia or anemia. The cDNAs for Epo have been cloned from many species. The mature proteins from the various species are highly conserved, exhibiting greater than 80% sequence identity at the amino acid level.

The biological effects of Epo are mediated by the Erythropoietin receptor (Epo R). At the protein sequence level, the human Epo R is approximately 83% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding truncated soluble forms of Epo R have been found. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

Entrez Gene IDs:

2056 (Human); 13856 (Mouse); 24335 (Rat)

Alternate Names:

ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2