Human Erythropoietin R Fluorescein-conjugated Antibody

Catalog #: FAB307F
2 Citations Datasheet / COA / SDS

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FAB307F

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Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry.

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Citations (2)

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Human Erythropoietin R Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed.

Source

Monoclonal Mouse IgG_2B Clone # 38409

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Pro26-Pro250
Accession # P19235

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB307F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Erythropoietin R

Erythropoietin R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Erythropoietin and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name

Erythropoietin Receptor

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Alternate Names

EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Erythropoietin R Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Erythropoietin mimetic compound AGEM400(HES) binds to the same receptor as erythropoietin but displays a different spectrum of activities.
Authors: Kessler C, Greindl A, Breuer B, Haberl U, Rybka A, Emgenbroich M, Frank HG, Potgens AJ
Cytokine, 2011-12-03;57(2):226-37.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Hypoxia-inducible factors in human pulmonary arterial hypertension: a link to the intrinsic myeloid abnormalities.
Authors: Farha S, Asosingh K, Xu W, Sharp J, George D, Comhair S, Park M, Tang WH, Loyd JE, Theil K, Tubbs R, Hsi E, Lichtin A, Erzurum SC
Blood, 2011-01-21;117(13):3485-93.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry