Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Western Blot, Flow Cytometry, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 38409
View all Erythropoietin R Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Pro26-Pro250
Accession # P19235

Specificity

Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Erythropoietin R Antibody

Detection of Human Erythropoietin R antibody by Western Blot.

Detection of Human Erythropoietin R by Western Blot.

Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Erythropoietin R antibody in TF-1 Human Cell Line antibody by Flow Cytometry.

Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry.

TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG Allophycocyanin-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of Human Erythropoietin R by Western Blot

Detection of Human Erythropoietin R by Western Blot

sEpoR characterization in uremic serum.1a. Soluble EpoR is detectable in serum from dialysis patients by western blot. Human serum was subjected to immunoprecipitation with goat anti-human erythropoietin receptor antibody (R&D Systems, AF-322-PB) followed by western blotting with mouse monoclonal anti-human erythropoietin receptor (R&D Systems, MAB307). Both antibodies recognize the extracellular domain of the receptor. Lanes 1–6 are serum from 6 representative dialysis patients, lane 7 is blank and lane 8 is recombinant sEpoR (Sigma Aldrich E0643, Saint Louis MI). Shown in the serum samples is a band of expected molecular weight of approximately 27 kDa. The control sEpoR with Fc tag is consistent with the manufacturers reported molecular weight of 32 kDa. 1b. Soluble EpoR is also detected using the same dialysis patient serum samples by performing immunoprecipitation in reverse order. In this experiment immunoprecipitation was done with mouse monoclonal anti-human erythropoietin receptor (R&D Systems, MAB307) followed by western blotting with goat anti-human erythropoietin receptor (R&D Systems, AF-322-PB). Lanes 1 to 3 are serum from 3 dialysis patients, and lane 4 is recombinant sEpoR-Fc (Sigma, 307) as positive control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/20169072), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Erythropoietin R by Western Blot

Detection of Mouse Erythropoietin R by Western Blot

The 59 kDa protein detected by M-20 In MCF-7 cells is not bound by other anti-EpoR antibodies.The indicated lysates were immunoprecipitated (IP) then the immunoblotted (IB) with the indicated antibodies: ab10653 (abcam Inc), Mab307 (R&D systems), C-20 & M-20 (Santa Cruz Inc) or A-82 (Amgen Inc). COS cell lysates expressing a FLAG-tagged version of EpoR (FLAG-EpoR) [6] and UT-7/Epo cells served as EpoR positive controls. 769-P cells served as the EpoR negative control. (A) Westerns were immunoprecipitated (IP) with ab10653 or M-20 followed by immunoblotting (IB) with M-20. The position of full-length 59 kDa EpoR in positive controls is indicated by the arrow. Positions of molecular weight markers (kDa) are shown. Bands detected in 769-P lysates are non-EpoR cross-reacting proteins and include antibody chains that were not removed completely or protein G that leached from beads. Note the detection of a 59 kDa band with MCF-7 cells with the M:20/M:20 combination but not with the ab10653/IB:M-20 combination. (B) IP:IB combinations with the indicated antibodies were subjected to western analysis. The western slice containing the 59 kDa EpoR band from each combination is shown. Note the 59 kDa bands detected in EpoR positive controls but not 769-P cells. Only the M-20:M-20 combination detected a 59 kDa band in MCF-7 cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0068083), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Erythropoietin R Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: TF-1 human erythroleukemic cell line

Immunoprecipitation

Khankin, E.V. et al. (2010) PLoS One 5: e9246.

Western Blot

2 µg/mL
Sample: UT‑7 human acute myeloid leukemia cell line

Reviewed Applications

Read 1 review rated 1 using MAB307 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name

Erythropoietin Receptor

Alternate Names

EpoR

Entrez Gene IDs

2057 (Human); 13857 (Mouse)

Gene Symbol

EPOR

UniProt

P19235

Additional Erythropoietin R Products

Product Documents for Human Erythropoietin R Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Erythropoietin R Antibody

For research use only

Citations for Human Erythropoietin R Antibody

Customer Reviews for Human Erythropoietin R Antibody (1)

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  • Human Erythropoietin R Antibody
    Name: Steve Elliott
    Application: Western Blot
    Sample Tested: UT-7 human acute myeloid leukemia cell line
    Species: Human
    Verified Customer | Posted 09/10/2016
    Sensitivity and specificity was assessed by western. Results indicate Mab307 cannot be used by western because of limited sensitivity to EpoR. By FLOW cytometry, MAB307 gave false-positive staining of negative control cell types.
    Human Erythropoietin R Antibody MAB307
    Bio-Techne Response
    R&D Systems Technical Service is following up.

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Protocols

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FAQs

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Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail