Detection of Human Erythropoietin R by Western Blot.
Western blot shows lysates of UT‑7 human acute myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin R
Epo R is a transmembrane protein expressed on the surface of
megakaryocytes, erythroid progenitors, and endothelial cells. It binds
Epo and transmits signals that stimulate the proliferation and
maturation of bone marrow erythroid precursors into red cells.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.
Western Blot: Human Erythropoietin R Antibody [MAB307].
UT-7 human acute myeloid leukemia cell line
Other Experimental Details
Other Experimental Details
Sensitivity and specificity was assessed by western. Results indicate Mab307 cannot be used by western because of limited sensitivity to EpoR. By FLOW cytometry, MAB307 gave false-positive staining of negative control cell types.R&D Systems Technical Service is following up.