|Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT‑7 human acute myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
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Western Blot: Human Erythropoietin R Antibody [MAB307]
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