Human Erythropoietin R Antibody

Catalog # Availability Size / Price Qty
Detection of Human Erythropoietin R by Western Blot.
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Product Details
Citations (4)
Supplemental Products
Reviews (1)

Human Erythropoietin R Antibody Summary

Species Reactivity
Detects human Erythropoietin R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Erythropoietin R is observed.
Monoclonal Mouse IgG2B Clone # 38409
Protein A or G purified from ascites
Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Accession # P19235
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
TF-1 human erythroleukemic cell line
Khankin, E.V. et al. (2010) PLoS One 5: e9246.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Erythropoietin R antibody by Western Blot. View Larger

Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Erythropoietin R antibody in TF-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG Allophycocyanin-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin R

Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.

Long Name
Erythropoietin Receptor
Entrez Gene IDs
2057 (Human); 13857 (Mouse)
Alternate Names
EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

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Citations for Human Erythropoietin R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissues.
    Authors: Patterson, Scott D, Rossi, John M, Paweletz, Katherin, Fitzpatrick, V Dan, Begley, C Glenn, Busse, Leigh, Elliott, Steve, McCaffery, Ian
    PLoS ONE, 2015;10(3):e0122149.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway.
    Authors: De Grandis, Maria, Cambot, Marie, Wautier, Marie-Pa, Cassinat, Bruno, Chomienne, Christin, Colin, Yves, Wautier, Jean-Luc, Le Van Kim, Caroline, El Nemer, Wassim
    Blood, 2013;121(4):658-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246.
    Species: Human
    Sample Types: Serum
    Applications: Immunoprecipitation
  4. Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
    Authors: Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
    J Neurosci Methods, 0;164(1):50-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Human Erythropoietin R Antibody
By Steve Elliott on 09/10/2016
Application: WB Sample Tested: UT-7 human acute myeloid leukemia cell line Species: Human

Sensitivity and specificity was assessed by western. Results indicate Mab307 cannot be used by western because of limited sensitivity to EpoR. By FLOW cytometry, MAB307 gave false-positive staining of negative control cell types.

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