Human Erythropoietin R Antibody
Human Erythropoietin R Antibody Summary
Pro26-Pro250
Accession # P19235
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Erythropoietin R by Western Blot. Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Erythropoietin R in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG Allophycocyanin-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin R
Epo R is a transmembrane protein expressed on the surface of megakaryocytes, erythroid progenitors, and endothelial cells. It binds Epo and transmits signals that stimulate the proliferation and maturation of bone marrow erythroid precursors into red cells.
Product Datasheets
Citations for Human Erythropoietin R Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissues.
Authors: Patterson, Scott D, Rossi, John M, Paweletz, Katherin, Fitzpatrick, V Dan, Begley, C Glenn, Busse, Leigh, Elliott, Steve, McCaffery, Ian
PLoS ONE, 2015;10(3):e0122149.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
JAK2V617F activates Lu/BCAM-mediated red cell adhesion in polycythemia vera through an EpoR-independent Rap1/Akt pathway.
Authors: De Grandis, Maria, Cambot, Marie, Wautier, Marie-Pa, Cassinat, Bruno, Chomienne, Christin, Colin, Yves, Wautier, Jean-Luc, Le Van Kim, Caroline, El Nemer, Wassim
Blood, 2013;121(4):658-65.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010;5(2):e9246.
Species: Human
Sample Types: Serum
Applications: Immunoprecipitation -
Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor.
Authors: Kirkeby A, van Beek J, Nielsen J, Leist M, Helboe L
J Neurosci Methods, 0;164(1):50-8.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Sensitivity and specificity was assessed by western. Results indicate Mab307 cannot be used by western because of limited sensitivity to EpoR. By FLOW cytometry, MAB307 gave false-positive staining of negative control cell types.
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