Human Fc gamma RIIIB/CD16b Antibody Summary
Accession # NP_000561
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Fc gamma RIIIB/CD16b in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) Rabbit Anti-Human Fc gamma RIIIB/CD16b Monoclonal Antibody (Catalog # MAB15971) or (B) Rabbit IgG isotype control antibody (MAB1050) followed by Goat anti-Rabbit IgG APC-conjugated Secondary Antibody (F0111) and Mouse Anti-Human Siglec-8 PE-conjugated Monoclonal Antibody (FAB7975P). Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fc gamma RIIIB/CD16b
Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.
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