Human Fc gamma  RIIIB/CD16b Antibody

R&D Systems | Catalog # MAB15971

Recombinant Monoclonal Antibody.
R&D Systems
Discontinued Product
MAB15971 has been discontinued. View all Fc gamma RIIIB/CD16b products.

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2830D
View all Fc gamma RIIIB/CD16b Primary Antibodies »

Product Specifications

Immunogen

HEK293-derived human Fc gamma RIIIB/CD16b protein
Met18-Ser200
Accession # NP_000561

Specificity

Detects human Fc gamma RIIIB/CD16b in Elisas.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Fc gamma  RIIIB/CD16b Antibody

Detection of Fc gamma RIIIB/CD16b in Human Peripheral Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with (A) Rabbit Anti-Human Fc gamma RIIIB/CD16b Monoclonal Antibody (Catalog # MAB15971) or (B) Rabbit IgG isotype control antibody (MAB1050) followed by Goat anti-Rabbit IgG APC-conjugated Secondary Antibody (F0111) and Mouse Anti-Human Siglec-8 PE-conjugated Monoclonal Antibody (FAB7975P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human Fc gamma  RIIIB/CD16b Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood cells

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIIB/CD16b

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name

Fc gamma Receptor III

Alternate Names

CD16b, FCG3, FCGR3B, FcgRIIIB, FcR-10, FCRIIIB, IGFR3

Entrez Gene IDs

2215 (Human); 14131 (Mouse)

Gene Symbol

FCGR3B

UniProt

NP_000561

Additional Fc gamma RIIIB/CD16b Products

Product Documents for Human Fc gamma  RIIIB/CD16b Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fc gamma  RIIIB/CD16b Antibody

For research use only

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