Key Product Details

Species Reactivity

Rabbit

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rabbit IgG

Specificity

Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rabbit IgG APC‑conjugated Antibody

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

Detection of Rabbit IgG Primary Antibody by Flow Cytometry

HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # AB-105-C), open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) at 10 µL/106cells.

Applications for Rabbit IgG APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Reviewed Applications

Read 3 reviews rated 5 using F0111 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rabbit IgG APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rabbit IgG APC‑conjugated Antibody

For research use only

Citations for Rabbit IgG APC‑conjugated Antibody

Customer Reviews for Rabbit IgG APC‑conjugated Antibody (3)

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3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Rabbit IgG APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 11/20/2020
    Rabbit IgG APC‑conjugated Antibody F0111
  • Rabbit IgG APC-conjugated Antibody
    Name: Danlee Enzler
    Application: ELISA
    Sample Tested: Serum
    Species: Rabbit
    Verified Customer | Posted 05/01/2018
  • Rabbit IgG APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Mouse
    Verified Customer | Posted 12/01/2017

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FAQs

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