Rabbit IgG APC-conjugated Antibody

Catalog # Availability Size / Price Qty
F0111
Detection of RabbitIgG Primary Antibody by Flow Cytometry
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Rabbit IgG APC-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of RabbitIgG Primary Antibody by Flow Cytometry View Larger

Detection of RabbitIgG Primary Antibody by Flow Cytometry HEK293 human embryonic kidney cell line transfected withV5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag MonoclonalAntibody (Catalog # MAB8926,filled histogram) or isotype control antibody
(Catalog # AB-105-C),open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgGSecondary Antibody
(Catalog # F0111) at 10 μL/106 cells. 

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citation for Rabbit IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice.
    Authors: Venet F, Huang X, Chung CS, Chen Y, Ayala A
    Am. J. Pathol., 2010;176(2):764-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Rabbit IgG APC-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Rabbit IgG APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Rabbit IgG APC-conjugated Antibody
By Anonymous on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Rabbit

Rabbit IgG APC-conjugated Antibody
By Anonymous on 12/01/2017
Application: Flow Sample Tested: Dendritic cells Species: Mouse