Rabbit IgG APC-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Rabbit IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (6)
Supplemental Products
Reviews (3)

Rabbit IgG APC-conjugated Antibody Summary

Species Reactivity
Rabbit IgG specific IgG was purified by rabbit IgG affinity chromatography.
Polyclonal Goat IgG
Antigen Affinity-purified
Rabbit IgG
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Flow Cytometry
Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rabbit IgG Primary Antibody by Flow Cytometry View Larger

Detection of Rabbit IgG Primary Antibody by Flow Cytometry HEK293 human embryonic kidney cell line transfected with V5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926, filled histogram) or isotype control antibody (Catalog # AB-105-C), open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) at 10 µL/106cells.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rabbit IgG APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents
    Authors: He, Y;Kaya, I;Shariatgorji, R;Lundkvist, J;Wahlberg, LU;Nilsson, A;Mamula, D;Kehr, J;Zareba-Paslawska, J;Biverstål, H;Chergui, K;Zhang, X;Andren, PE;Svenningsson, P;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair
    Authors: JA Lindborg, NM Tran, DM Chenette, K DeLuca, Y Foli, R Kannan, Y Sekine, X Wang, M Wollan, IJ Kim, JR Sanes, SM Strittmatt
    Cell Reports, 2021-03-02;34(9):108777.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CD4+ T Cell-Derived NGAL Modifies the Outcome of Ischemic Acute Kidney Injury
    Authors: SA Lee, S Noel, JT Kurzhagen, M Sadasivam, PM Pierorazio, LJ Arend, AR Hamad, H Rabb
    J. Immunol., 2019-12-30;204(3):586-595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Heparin Forms Polymers with Cell-free DNA Which Elongate Under Shear in Flowing Blood
    Authors: JC de Vries, AD Barendrech, CC Clark, RT Urbanus, P Boross, S de Maat, C Maas
    Sci Rep, 2019-12-04;9(1):18316.
    Species: Human
    Sample Types: Citrated Blood
    Applications: IHC
  5. Modeling the Pathological Long-Range Regulatory Effects of Human Structural Variation with Patient-Specific hiPSCs
    Authors: M Laugsch, M Bartusel, R Rehimi, H Alirzayeva, A Karaolidou, G Crispatzu, P Zentis, M Nikolic, T Bleckwehl, P Kolovos, WFJ van Ijcken, T Šari?, K Koehler, P Frommolt, K Lachlan, J Baptista, A Rada-Igles
    Cell Stem Cell, 2019-04-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Plasmacytoid dendritic cells control lung inflammation and monocyte recruitment in indirect acute lung injury in mice.
    Authors: Venet F, Huang X, Chung CS, Chen Y, Ayala A
    Am. J. Pathol., 2009-12-30;176(2):764-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Reviews for Rabbit IgG APC-conjugated Antibody

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Rabbit IgG APC-conjugated Antibody
By Anonymous on 11/20/2020
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Rabbit IgG APC-conjugated Antibody
By Danlee Enzler on 05/01/2018
Application: ELISA Sample Tested: Serum Species: Rabbit

Rabbit IgG APC-conjugated Antibody
By Anonymous on 12/01/2017
Application: Flow Sample Tested: Dendritic cells Species: Mouse