Detection of RabbitIgG Primary Antibody by Flow Cytometry HEK293 human embryonic kidney cell line transfected withV5-tagged proteins was stained with Rabbit Anti-V5 Epitope Tag MonoclonalAntibody (Catalog # MAB8926,filled histogram) or isotype control antibody |
(Catalog # AB-105-C),open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgGSecondary Antibody
(Catalog # F0111) at 10 μL/106 cells.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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We have 1 review tested in 1 species: Mouse.We have 1 review tested in 1 application: Flow Cytometry.