Detection of RabbitIgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) werestained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody(Catalog # FAB4867A)and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog #MAB3487)or |
(B) Normal Rabbit IgG Control (Catalog # MAB1050) followed byPhycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0110).To facilitate intracellular staining, cells were fixed and permeabilized withFlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #FC012).View our protocol for StainingIntracellular Molecules.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.