Normal Rabbit IgG Control Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of RabbitIgG Control by Flow Cytometry
Human peripheral blood mononuclear cells (PBMCs) werestained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody(Catalog # FAB4867A)and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog #MAB3487)or
(B) Normal Rabbit IgG Control (Catalog # MAB1050) followed byPhycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0110).To facilitate intracellular staining, cells were fixed and permeabilized withFlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #FC012).View our protocol for StainingIntracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
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