Normal Rabbit IgG Control

Recombinant Monoclonal Isotype Control.
  
  • Specificity
    Selected for use as rabbit IgG isotype control for flow cytometry applications.
  • Source
    Monoclonal Rabbit IgG Clone # 60024B
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    KLH
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of RabbitIgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) werestained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody(Catalog # FAB4867A)and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog #MAB3487)or
(B) Normal Rabbit IgG Control (Catalog # MAB1050) followed byPhycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0110).To facilitate intracellular staining, cells were fixed and permeabilized withFlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #FC012).View our protocol for StainingIntracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 40  
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Normal Rabbit IgG Alexa Fluor® 488-conjugated Control

Ctrl IC105G
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Normal Rabbit IgG PE-conjugated Control

Ctrl IC105P
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Rabbit IgG PE-conjugated Antibody

Ctrl IC1051P
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Rabbit IgG Alexa Fluor® 647-conjugated Antibody

Ctrl IC1051R
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Normal Rabbit IgG APC-conjugated Control

Ctrl IC105A
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Rabbit IgG Alexa Fluor® 488-conjugated Antibody

Ctrl IC1051G
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Rabbit IgG PerCP-conjugated Antibody

Ctrl IC1051C
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Rabbit IgG Alexa Fluor® 700-conjugated Antibody

Ctrl IC1051N
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Rabbit IgG Fluorescein-conjugated Antibody

Ctrl IC1051F
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Rabbit IgG Alexa Fluor® 405-conjugated Antibody

Ctrl IC1051V
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Rabbit IgG APC-conjugated Antibody

Ctrl IC1051A
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Rabbit IgG Alexa Fluor® 750-conjugated Antibody

Ctrl IC1051S
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Rabbit IgG Fluorescein‑conjugated Control Antibody

Ctrl IC105F
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Rabbit IgG Alexa Fluor® 594-conjugated Antibody

Ctrl IC1051T
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Normal Rabbit IgG Alexa Fluor® 700-conjugated Control

Ctrl IC105N
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