Normal Rabbit IgG Control

Recombinant Monoclonal Isotype Control
Catalog # Availability Size / Price Qty
MAB1050-500
Detection of RabbitIgG Control by Flow Cytometry
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Normal Rabbit IgG Control Summary

Specificity
Selected for use as rabbit IgG isotype control for flow cytometry applications.
Source
Monoclonal Rabbit IgG Clone # 60024B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
KLH
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Detection of RabbitIgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) werestained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody(Catalog # FAB4867A)and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog #MAB3487)or
(B) Normal Rabbit IgG Control (Catalog # MAB1050) followed byPhycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #F0110).To facilitate intracellular staining, cells were fixed and permeabilized withFlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #FC012).View our protocol for StainingIntracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

FAQs

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