Human FCRL3/FcRH3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human FCRL3/FcRH3 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FCRL2 and rhFCRL5 is observed, and no cross-reactivity with rhFCRL1 or recombinant mouse FCRL3 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 546828
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FCRL3/FcRH3
    Accession # Q96P31
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of FCRL3/FcRH3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human FCRL3/FcRH3 APC‑conjugated Monoclonal Antibody (Catalog # FAB3126A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FCRL3/FcRH3

FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2-4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).

  • References:
    1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
    2. Miller, I. et al. (2002) Blood, 99:2662.
    3. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
    4. Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
    5. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
    6. Kochi, Y. et al. (2005) Nat. Genet. 37:478.
    7. Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.
  • Long Name:
    Fc Receptor-like 3
  • Entrez Gene IDs:
    115352 (Human); 329693 (Mouse); 680697 (Rat)
  • Alternate Names:
    CD307c; Fc receptor homolog 3; Fc receptor-like 3; Fc receptor-like protein 3; FcRH3; fcR-like protein 3; hIFGP3; IFGP3; immunoglobulin superfamily receptor translocation associated protein 3; IRTA3; SPAP2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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