Human NCAM-1/CD56 PE-conjugated Antibody

(4 citations)   
  • Species Reactivity
  • Specificity
    Detects human NCAM‑1/CD56 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM-1-L1, recombinant mouse (rm) MAdCAM-1, rmNCAM-1-L1, or rmOCAM is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 301040
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NCAM‑1/CD56
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NCAM‑1/CD56 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NCAM-1/CD56

Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans (1), the extracellular matrix protein agrin (2), and several chondroitin sulfate proteoglycans that include neurocan and phosphocan (3). There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins (4‑6). Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180 (7‑8). NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment (4). The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features (9). NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity (10‑13).

  • References:
    1. Burg, M.A. et al. (1995) J. Neurosci. Res. 41:49.
    2. Storms, S.D. and U. Rutishauser (1998) J Biol. Chem. 273:27124.
    3. Margolis, R.K. et al. (1996) Perspect. Dev. Neurobiol. 3:273.
    4. Dickson, G. et al. (1987) Cell 50:1119.
    5. Lanier, L.L. et al. (1991) J. Immunol. 146:4421.
    6. Hemperly, J.J. et al. (1990) J. Mol. Neurosci. 2:71.
    7. Rutishauser, U.and C. Goridis (1986) Trends Genet. 2:72.
    8. Vawter, M.P. et al. (2001) Exp. Neurol. 172:29.
    9. Rutihauser, U. (1990) Adv. Exp. Med. Biol. 265:179.
    10. Becker, C.G. et al. (1996) J. Neurosci. Res. 45:143.
    11. Doherty, P. et al. (1995) J. Neurobiol. 26:437.
    12. Eckardt, M. et al. (2000) J. Neurosci. 20:5234.
    13. Muller, D. et al. (1996) Neuron 17:413.
  • Long Name:
    Neural Cell Adhesion Molecule
  • Entrez Gene IDs:
    4684 (Human); 17967 (Mouse); 24586 (Rat)
  • Alternate Names:
    CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Hepatic progenitor cells express SerpinB3.
    Authors: Villano G, Turato C, Quarta S, Ruvoletto M, Ciscato F, Terrin L, Semeraro R, Paternostro C, Parola M, Alvaro D, Bernardi P, Gatta A, Pontisso P
    BMC Cell Biol, 2014;15(0):5.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Natural killer lysis receptor (NKLR)/NKLR-ligand matching as a novel approach for enhancing anti-tumor activity of allogeneic NK cells.
    Authors: Markel G, Seidman R, Besser MJ
    PLoS ONE, 2009;4(5):e5597.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions.
    Authors: Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR
    FASEB J., 2007;21(10):2442-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Cytokines induced during chronic hepatitis B virus infection promote a pathway for NK cell-mediated liver damage.
    Authors: Dunn C, Brunetto M, Reynolds G, Christophides T, Kennedy PT, Lampertico P, Das A, Lopes AR, Borrow P, Williams K, Humphreys E, Afford S, Adams DH, Bertoletti A, Maini MK
    J. Exp. Med., 2007;204(3):667-80.
    Species: Human
    Sample Type: Serum
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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