Human FCRL5/FCRL3 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human FCRL5/FCRL3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) FCRL3 is observed and no cross-reactivity with rhFCRL1, 2, or 4 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 307307
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FCRL5/FCRL3
    Accession # AAI01067
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of FCRL5/FCRL3 in CD19+ Human Lymphocytes by Flow Cytometry. CD19+ human lymphocytes were stained with Mouse Anti-Human FCRL5/FCRL3 PE‑conjugated Monoclonal Antibody (Catalog # FAB20871P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FCRL5/FCRL3

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one Immunotyrosine Activation Motif (ITAM) and two Immunotyrosine Inhibitory Motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5-7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the up-regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is up-regulated on tumor cells in some types of B cell malignancies (6, 10-12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

  • References:
    1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
    2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
    3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
    4. SwissProt # Q96RD9.
    5. Miller, I. et al. (2002) Blood 99:2662.
    6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
    7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
    8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
    9. Mohan, J. et al. (2006) Blood 107:4433.
    10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
    11. Ise, T. et al. (2007) Leukemia 21:169.
    12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
    13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.
  • Long Name:
    Fc Receptor-like 5/3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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