Human FCRL5/FCRL3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FCRL5/FCRL3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) FCRL3 is observed and no cross-reactivity with rhFCRL1, 2, or 4 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 307307
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FCRL5/FCRL3
    Gln16-Arg844
    Accession # AAI01067
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of FCRL5/FCRL3 in Human CD19+ Lymphocytes by Flow Cytometry. Human blood‑derived CD19+ lymphocytes were stained with Mouse Anti-Human FCRL5/FCRL3 Monoclonal Antibody (Catalog # MAB20871, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FCRL5/FCRL3

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one immunotyrosine activation motif (ITAM) and two immunotyrosine inhibitory motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5‑7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the up‑regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is up‑regulated on tumor cells in some types of B cell malignancies (6, 10‑12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

  • References:
    1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
    2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
    3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
    4. SwissProt # Q96RD9.
    5. Miller, I. et al. (2002) Blood 99:2662.
    6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
    7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
    8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
    9. Mohan, J. et al. (2006) Blood 107:4433.
    10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
    11. Ise, T. et al. (2007) Leukemia 21:169.
    12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
    13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.
  • Long Name:
    Fc Receptor-like 5/3
  • Alternate Names:
    FCRL5/FCRL3
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Mouse IgG2B Antibody

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Rat Anti-Mouse IgG2B PE-conjugated Antibody

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