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Human Fetuin A Quantikine ELISA Kit

R&D Systems | Catalog # DFTA00

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

1.74 ng/mL

Assay Range

7.8-500 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all Fetuin A/AHSG ELISA Kits »

Product Summary for Human Fetuin A Quantikine ELISA Kit

The Quantikine Human Fetuin A Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fetuin A in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Fetuin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fetuin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fetuin A.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Fetuin A

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 45.0 147 344 47.1 149 334
Standard Deviation 3.32 10.6 19.6 3.00 10.7 28.2
CV% 7.4 7.2 5.7 6.4 7.2 8.5

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 43.3 124 264 44.9 127 269
Standard Deviation 1.69 6.08 10.6 3.67 9.34 22.6
CV% 3.9 4.9 4.0 8.2 7.3 8.4

Recovery for Human Fetuin A Quantikine ELISA Kit

The recovery of Fetuin A spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 93-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Fetuin A were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Fetuin A/AHSG ELISA Linearity

Scientific Data Images for Human Fetuin A Quantikine ELISA Kit

Human Fetuin A/AHSG ELISA Cell Culture Supernate Standard Curve

Human Fetuin A/AHSG ELISA Cell Culture Supernate Standard Curve

Human Fetuin A/AHSG ELISA Serum/Plasma Standard Curve

Human Fetuin A/AHSG ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fetuin A/AHSG

Fetuins are members of the cystatin family of proteases. Secreted by the liver, fetuins can be found at high levels in serum. Fetuin A promotes phagocytosis of apoptotic cells and plays a role in bone development, calcium homeostasis and insulin sensitivity. Fetuin B functions as an inhibitor of basic calcium phosphate precipitation.

Long Name

alpha-2-HS Glycoprotein

Alternate Names

AHSG, alpha-2-HS-glycoprotein

Entrez Gene IDs

197 (Human); 11625 (Mouse); 25373 (Rat)

Gene Symbol

AHSG

Additional Fetuin A/AHSG Products

Product Documents for Human Fetuin A Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Fetuin A Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Fetuin A Quantikine ELISA Kit

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Protocols

View specific protocols for Human Fetuin A Quantikine ELISA Kit (DFTA00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of the appropriate Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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