• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    1.74 ng/mL
  • Assay Range
    7.8 - 500 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Fetuin A
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC90, Quantikine Immunoassay Control Set 689 for Human Fetuin A - Please Inquire
Product Summary
The Quantikine Human Fetuin A Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fetuin A in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Fetuin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fetuin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fetuin A.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean4514734447.1149334
Standard Deviation3.3210.619.6310.728.2
CV%7.47.25.76.47.28.5

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean43.312426444.9127269
Standard Deviation1.696.0810.63.679.3422.6
CV%3.94.948.27.38.4

Recovery

The recovery of Fetuin A spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 93-109
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Fetuin A were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Fetuin A Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Fetuin A/AHSG
Fetuins are members of the cystatin family of proteases. Secreted by the liver, fetuins can be found at high levels in serum. Fetuin A promotes phagocytosis of apoptotic cells and plays a role in bone development, calcium homeostasis and insulin sensitivity. Fetuin B functions as an inhibitor of basic calcium phosphate precipitation.
    • Long Name
      alpha-2-HS Glycoprotein
    • Entrez Gene IDs
      197 (Human); 11625 (Mouse); 25373 (Rat);
    • Alternate Names
      A2HS; AHS; AHSG; alpha-2-HS-glycoprotein; alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUAba-alpha-2-glycoprotein; Fetuin A; fetuin-A; HSGA;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of the appropriate Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    7 Citations: Showing 1 - 7
    Filter your results:

    Species
    Sample Type
    1. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
      Int J Mol Sci, 2016;17(8):.
      Species: Human
      Sample Type: Serum
    2. Proteomic Biomarkers Panel: New Insights in Chronic Kidney Disease
      Dis Markers, 2016;2016(0):3185232.
      Species: Human
      Sample Type: Serum
    3. Plasma levels of fetuin-A and hepatic enzymes and risk of type 2 diabetes in women in the U.S.
      Authors: Sun Q, Cornelis M, Manson J, Hu F
      Diabetes, 2013;62(1):49-55.
      Species: Human
      Sample Type: Plasma
    4. Serum fetuin-A levels are associated with the presence and severity of coronary artery disease in patients with type 2 diabetes.
      Biomarkers, 2013;18(2):160-4.
      Species: Human
      Sample Type: Serum
    5. Association between higher serum fetuin-A concentrations and abnormal albuminuria in middle-aged and elderly chinese with normal glucose tolerance.
      Authors: Li M, Xu M, Bi Y
      Diabetes Care, 2010;33(11):2462-4.
      Species: Human
      Sample Type: Serum
    6. NF-kappaB mediates lipid-induced fetuin-A expression in hepatocytes that impairs adipocyte function effecting insulin resistance.
      Authors: Dasgupta S, Bhattacharya S, Biswas A
      Biochem. J., 2010;429(3):451-62.
      Species: Human
      Sample Type: Serum
    7. The influence of vitamin D analogs on calcification modulators, N-terminal pro-B-type natriuretic peptide and inflammatory markers in hemodialysis patients: a randomized crossover study.
      Authors: Hansen D, Rasmussen K, Rasmussen L, Bruunsgaard H, Brandi L
      BMC Nephrol, 0;15(0):130.
      Species: Human
      Sample Type: Plasma
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 689 for Human Fetuin A

    Ctrl QC90
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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