Human FGF-19 Quantikine ELISA Kit

  (10 citations)
(3 Reviews)
    
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Assay Procedure
Citations (10)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    3.35 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human FGF-19
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC24 , Quantikine Immunoassay Control Group 9 - Please Inquire
Product Summary
The Quantikine Human FGF-19 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FGF-19 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF-19 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FGF-19 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FGF-19.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 113 346 689 119 349 700
Standard Deviation 7.26 15.7 24.8 6.46 19.3 31.7
CV% 6.4 4.5 3.6 5.5 5.5 4.5

Recovery

The recovery of FGF-19 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 104 93-109
EDTA Plasma (n=4) 94 85-101
Heparin Plasma (n=4) 92 89-95
Serum (n=4) 95 88-100
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of FGF-19 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 FGF-19 [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FGF-19
FGF-19 (Fibroblast Growth Factor-19) is a secreted heparin-binding protein in the endocrine subfamily of FGFs that also includes FGF-21 and FGF-23. It is produced by epithelial cells of the ileal intestine in response to bile acids and signals through a receptor complex of FGF R4 and Klotho-beta in the liver. FGF-19 suppresses bile acid synthesis and enhances hepatic protein and glycogen synthesis. Dysregulalted FGF-19 activity is associated with the development of hypercholesterolemia, gall stones, bile acid induced diarrhoea (BAD), hepatocellular cancer, colon cancer, and metabolic syndrome.
  • Long Name:
    Fibroblast Growth Factor 19
  • Entrez Gene IDs:
    9965 (Human)
  • Alternate Names:
    FGF19; FGF-19; fibroblast growth factor 19
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Sample Type
  1. Impact of gut hormone FGF-19 on type-2 diabetes and mitochondrial recovery in a prospective study of obese diabetic women undergoing bariatric surgery
    Authors: L Martinez d, I Kyrou, J Vrbikova, V Hainer, P Sramkova, M Fried, MK Piya, S Kumar, G Tripathi, PG McTernan
    BMC Med, 2017;15(1):34.
    Species: Human
    Sample Type: Serum
  2. Potential mechanisms of effects of serum-derived bovine immunoglobulin/protein isolate therapy in patients with diarrhea-predominant irritable bowel syndrome
    Authors: N Valentin, M Camilleri, P Carlson, SC Harrington, D Eckert, J O'Neill, D Burton, J Chen, AL Shaw, A Acosta
    Physiol Rep, 2017;5(5):.
    Species: Human
    Sample Type: Serum
  3. Acute caloric restriction counteracts hepatic bile acid and cholesterol deficiency in morbid obesity
    Authors: S Straniero, F Rosqvist, D Edholm, H Ahlström, J Kullberg, M Sundbom, U Risérus, M Rudling
    J. Intern. Med, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  4. Serum bile acids and GLP-1 decrease following telemetric induced weight loss: results of a randomized controlled trial
    Sci Rep, 2016;6(0):30173.
    Species: Human
    Sample Type: Plasma
  5. Bile Acids and Dysbiosis in Non-Alcoholic Fatty Liver Disease
    PLoS ONE, 2016;11(5):e0151829.
    Species: Human
    Sample Type: Serum
  6. Modulation of fibroblast growth factor 19 expression by bile acids, meal replacement and energy drinks, milk, and coffee.
    Authors: Styer, Amanda M, Roesch, Stephen, Argyropoulos, George
    PLoS ONE, 2014;9(1):e85558.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Serum concentrations of fibroblast growth factors 19 and 21 in women with gestational diabetes mellitus: association with insulin resistance, adiponectin, and polycystic ovary syndrome history.
    Authors: Wang D, Zhu W, Li J, An C, Wang Z
    PLoS ONE, 2013;8(11):e81190.
    Species: Human
    Sample Type: Serum
  8. Endocrine fibroblast growth factor FGF19 promotes prostate cancer progression.
    Authors: Feng S, Dakhova O, Creighton C, Ittmann M
    Cancer Res, 2013;73(8):2551-62.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. The role of bile after Roux-en-Y gastric bypass in promoting weight loss and improving glycaemic control.
    Authors: Pournaras, Dimitri, Glicksman, Clare, Vincent, Royce P, Kuganolipava, Shophia, Alaghband-Zadeh, Jamie, Mahon, David, Bekker, Jan H R, Ghatei, Mohammad, Bloom, Stephen, Walters, Julian R, Welbourn, Richard, le Roux, Carel W
    Endocrinology, 2012;153(8):3613-9.
    Species: Human
    Sample Type: Plasma
  10. Bile acids activate fibroblast growth factor 19 signaling in human hepatocytes to inhibit cholesterol 7alpha-hydroxylase gene expression.
    Authors: Song KH, Li T, Owsley E
    Hepatology, 2009;49(1):297-305.
    Species: Human
    Sample Type: Cell Culture Supernates

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Average Rating: 5 (Based on 3 reviews)

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  Excellent
  Anonymous 12/01/2017
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Sample Tested 2120Ep human embryonal carcinoma cell line
  Excellent
  Anonymous 10/23/2017
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Sample Tested EDTA Plasma

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Other Experimental Details solid assay
  Excellent
  Anonymous 09/30/2016
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Sample Tested pig serum

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Other Experimental Details it was quick, easy and clear result. standards gave well standard curve, quite sensitive

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