Human FGF R2 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects alpha isoforms (3 Ig-like domains) and beta isoforms (2 Ig-like domains) of human FGF R2 in direct ELISAs and Western blots. In direct ELISAs, this antibody reacts with all isoforms of rhFGF R2 but shows approximately a 4-fold preference for (IIIc) isoforms. Approximately 100% cross-reactivity with recombinant mouse FGF R2, 15% cross-reactivity with recombinant human (rh) FGF RI ( beta  isoforms), and no cross-reactivity with rhFGF RI ( alpha isoforms), rhFGF R3, or rhFGF R4 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 98725
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of FGF R2 in KATO-III Human Cell Line by Flow Cytometry. KATO-III human gastric carcinoma cell line was stained with Mouse Anti-Human FGF R2 APC-conjugated Monoclonal Antibody (Catalog # FAB684A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FGF R2

FGF R2 is a transmembrane tyrosine kinase that functions as a receptor for several FGF family proteins. The extracellular domain includes three ( alpha  isoforms) or two ( beta  isoforms) Ig-like domains. Alternative exon usage results in IIIb or IIIc isoforms that differ in their membrane proximal Ig-like domain.

  • Long Name:
    Fibroblast Growth Factor Receptor 2
  • Entrez Gene IDs:
    2263 (Human); 14183 (Mouse)
  • Alternate Names:
    BFR-1; CD332 antigen; CD332; CFD1; craniofacial dysostosis 1; EC 2.7.10; EC; FGFR2; fibroblast growth factor receptor 2; FLJ98662; Jackson-Weiss syndrome; JWS; Keratinocyte growth factor receptorreceptor like 14; KSAM; K-sam; soluble FGFR4 variant 4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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