Forkhead Box P1 (FOXP1) is a member of the FOX family of transcription factors. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 contains both a DNA binding domain as well as protein-protein interaction domains. FoxP1 can homo or heterodimerize with FoxP2 and FoxP4, with dimerization necessary for DNA binding. FoxP1 shows both oncogenic and tumor suppressive characteristics. Overexpression in lymphomas leads to poor prognosis, but loss of FoxP1 in breast cancer also implicates a poor prognosis. Human isoforms of 489 to 677 amino acids contain alternate sequences within the first 60 amino acids and/or deletion of amino acids.
Human FoxP1 APC‑conjugated Antibody
R&D Systems | Catalog # IC45341A
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 837016
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Product Specifications
Immunogen
E. coli-derived recombinant human FoxP1
Lys548-Glu677
Accession # Q9H334
Lys548-Glu677
Accession # Q9H334
Specificity
Detects human FoxP1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human FoxP1 APC‑conjugated Antibody
Detection of FoxP1 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FoxP1 APC-conjugated Monoclonal Antibody (Catalog # IC45341A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.Applications for Human FoxP1 APC‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line fixed with 4% paraformaldehyde and permeabilized with methanol
Sample: MCF‑7 human breast cancer cell line fixed with 4% paraformaldehyde and permeabilized with methanol
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FoxP1
Long Name
Forkhead Box P1
Alternate Names
QRF1
Gene Symbol
FOXP1
UniProt
Additional FoxP1 Products
Product Documents for Human FoxP1 APC‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FoxP1 APC‑conjugated Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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