Human FoxP1 Antibody

Detection of FoxP1 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with methanol.

Detection of Human FoxP1 by Simple Western^TM.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.5 mg/mL. A specific band was detected for FoxP1 at approximately 99 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB45341). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human FoxP1 by Immunocytochemistry/Immunofluorescence

Spinal organoids consists of various spinal cord cell types.Representative images illustrating the presence of a HOXB4+ and b HOXC8+ cells in spinal organoids. Cellular nuclei were counterstained with DAPI. Scale bars, 100 μm. c Co-staining of FOXP1 (green) and ISL1 (red) demonstrates presence of limb-innervating neurons in spinal organoids. Scale bars, 100 μm. d Representative images of spinal organoids at day 42 stained with ISL1 (red) and ChAT (green). Cellular nuclei were counterstained with DAPI. Scale bars, 100 μm. Spinal organoids are stained with e CHX10+ cells (RED) and f CALB+ cells (green). Scale bars, 100 μm. g Co-staining of S100 beta and TUJ1 shows presence of astrocytes in spinal organoids. Scale bars, 100 μm. h Quantitative-PCR analysis demonstrates a lack of dorsal cell types in the spinal organoids generated Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30368521), licensed under a CC-BY license. Not internally tested by R&D Systems.