Human FSHR APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB65591A

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Detection of FSH R in OVCAR‑3 Human Cell Line by Flow Cytometry.
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Human FSHR APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FSH R in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 626717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FSH R
Accession # M65085
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of FSH R antibody in OVCAR-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of FSH R in OVCAR‑3 Human Cell Line by Flow Cytometry. OVCAR-3 human ovarian carcinoma cell line was stained with Mouse Anti-Human FSH R APC-conjugated Monoclonal Antibody (Catalog # FAB65591A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of FSH R antibody in HEK293 Human Cell Line Transfected with Human FSH R and eGFP antibody by Flow Cytometry. View Larger

Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human FSH R and eGFP was stained with Mouse Anti-Human FSH R APC-conjugated Monoclonal Antibody (Catalog # FAB65591A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FSHR

Follicle Stimulating Hormone Receptor (FSH R), also called Follitropin Receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Long Name
Follicle Stimulating Hormone Receptor
Entrez Gene IDs
2492 (Human); 14309 (Mouse)
Alternate Names
follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH R; FSH receptor; FSHR; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1

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