Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 626717
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Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human FSH R
Accession # M65085

Specificity

Detects human FSH R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human FSHR APC-conjugated Antibody

Detection of FSH R antibody in OVCAR-3 Human Cell Line antibody by Flow Cytometry.

Detection of FSH R in OVCAR‑3 Human Cell Line by Flow Cytometry.

OVCAR-3 human ovarian carcinoma cell line was stained with Mouse Anti-Human FSH R APC-conjugated Monoclonal Antibody (Catalog # FAB65591A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of FSH R antibody in HEK293 Human Cell Line Transfected with Human FSH R and eGFP antibody by Flow Cytometry.

Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human FSH R and eGFP was stained with Mouse Anti-Human FSH R APC-conjugated Monoclonal Antibody (Catalog # FAB65591A). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human FSHR APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: OVCAR‑3 human ovarian carcinoma cell line and HEK293 human embryonic kidney cell line transfected with human FSH R and eGFP

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FSHR

Follicle Stimulating Hormone Receptor (FSH R), also called Follitropin Receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Long Name

Follicle Stimulating Hormone Receptor

Alternate Names

FSH R, LGR1, ODG1

Entrez Gene IDs

2492 (Human); 14309 (Mouse)

Gene Symbol

FSHR

UniProt

Additional FSHR Products

Product Documents for Human FSHR APC-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FSHR APC-conjugated Antibody

For research use only

Citations for Human FSHR APC-conjugated Antibody

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