Human FSHR Antibody

Catalog #: MAB65591
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB65591
MAB65591-SP

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Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FSHR Antibody Summary

Species Reactivity

Human

Specificity

Detects human FSH R in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 626717

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human FSH R
Accession # M65085

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize FSH alpha / beta -induced cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The Neutralization Dose (ND₅₀) is typically 0.02-0.1 µg/mL in the presence of 1 ng/mL Recombinant Human FSH alpha / beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FSH R or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591) followed by APC-conjugated Goat anti-Mouse Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Neutralization

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cAMP Production Induced by FSH alpha / beta and Neutralization by Human FSH R Antibody. Recombinant Human FSH a/ beta (Catalog # 5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recom-binant Human FSH a/ beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND₅₀ is typically 0.02-0.1 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FSHR

Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Long Name

Follicle Stimulating Hormone Receptor

Entrez Gene IDs

2492 (Human); 14309 (Mouse)

Alternate Names

follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH R; FSH receptor; FSHR; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1

Product Datasheets

Product Datasheet

COA

SDS

Citation for Human FSHR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways
Authors: F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol
Oncogene, 2017-09-18;37(3):286-301.
Species: Human
Sample Types: Whole Cells
Applications: ICC