Human FSHR Antibody

Catalog # Availability Size / Price Qty
MAB65591
MAB65591-SP
Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry.
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Product Details
Citations (1)
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Human FSHR Antibody Summary

Species Reactivity
Human
Specificity
Detects human FSH R in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 626717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FSH R
Accession # M65085
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize FSH alpha / beta -induced cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R. Morgenthaler, N.G. et al. (1998) Horm. Metab. Res. 30:162. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 1 ng/mL Recombinant Human FSH alpha / beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of FSH R antibody in HEK293 Human Cell Line Transfected with Human FSH R and eGFP antibody by Flow Cytometry. View Larger

Detection of FSH R in HEK293 Human Cell Line Transfected with Human FSH R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human FSH R or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591) followed by APC-conjugated Goat anti-Mouse Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Neutralization cAMP Production Induced by FSH  alpha / beta  and Neutralization by Human FSH R Antibody. View Larger

cAMP Production Induced by FSH alpha / beta and Neutralization by Human FSH R Antibody. Recombinant Human FSH a/ beta (Catalog # 5925-FS) induces cAMP production in the HEK293 human embryonic kidney cell line transfected with human FSH R in a dose-dependent manner (orange line), as measured by the cAMP Parameter Assay Kit (Catalog # KGE002B). cAMP production elicited by Recom-binant Human FSH a/ beta (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FSH R Monoclonal Antibody (Catalog # MAB65591). The ND50 is typically 0.02-0.1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FSHR

Follicle stimulating hormone receptor (FSH R), also called follitropin receptor or LGR1, is a 695 amino acid (aa), ~87 kDa 7-transmembrane receptor of the leucine-rich repeat glycoprotein hormone receptor subfamily of GPCRs. It is expressed by ovarian granulosa cells and is essential for ovarian follicle maturation. In the male, it is expressed by Sertoli cells and plays a minor role in male fertility. Human FSH R shares 86% aa sequence identity with mouse and rat FSH R within the 349 aa N‑terminal extracellular domain. A 754 aa isoform contains an inserted sequence at aa 75.

Long Name
Follicle Stimulating Hormone Receptor
Entrez Gene IDs
2492 (Human); 14309 (Mouse)
Alternate Names
follicle stimulating hormone receptor; follicle-stimulating hormone receptor; Follitropin receptor; FSH R; FSH receptor; FSHR; FSH-R; LGR1; LGR1FSHRO; MGC141667; MGC141668; ODG1

Product Datasheets

Citation for Human FSHR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways
    Authors: F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol
    Oncogene, 2018;37(3):286-301.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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